外源乙烯对冷藏桃果实香气物质合成的调控

doi:10.11707/j.1001-7488.20140308

林业科学 ›› 2014, Vol. 50 ›› Issue (3): 55-62.doi: 10.11707/j.1001-7488.20140308

外源乙烯对冷藏桃果实香气物质合成的调控

王贵章¹, 李杨昕², 王贵禧¹, 梁丽松¹, 马庆华¹

1. 林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所北京 100091;
2. 北京市建华实验学校北京 100039

收稿日期:2013-02-28 修回日期:2013-12-23 出版日期:2014-03-25 发布日期:2014-04-16
基金资助:
国家自然科学基金项目（31071834）。

Aroma Volatiles Biosynthesis and Relative Enzyme Activities Regulated by Exogenous Ethylene in Peach Fruits Stored at Low Temperature

Wang Guizhang¹, Li Yangxin², Wang Guixi¹, Liang Lisong¹, Ma Qinghua¹

1. State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091;
2. Beijing Jianhua Experimental School Beijing 100039

Received:2013-02-28 Revised:2013-12-23 Online:2014-03-25 Published:2014-04-16
Contact: 王贵禧

摘要/Abstract

摘要：

为了探讨外源乙烯对冷藏过程中‘大久保’桃果实香气合成的调控，以常温贮藏桃（CK1）和0 ℃贮藏桃（CK2）为对照，在低温贮藏期间外源乙烯处理（T）对桃果实中芳香成分的释放及香气合成相关酶脂氧合酶（LOX）、乙醇脱氢酶（ADH）和乙醇酰基转移酶（AAT）活性的影响进行研究。结果表明：在测得的29种挥发性香气中，有19种成分的合成受到低温抑制，含量降低，在外源乙烯的诱导下除了薄荷醇外，其余18种成分含量均升高；在10种受低温诱导而含量增加的成分中，有4种同时被外源乙烯诱导，而其余6种成分则受到外源乙烯的抑制含量降低。低温阻遏薄荷醇的合成，因此只在常温条件下检测到。低温冷藏导致花香型香气酯类物质代谢发生障碍，果实品质下降；而施加外源乙烯能促进特征香气芳樟醇和γ-癸内酯的合成，抑制己醛、反-2-己烯醛和苯甲醛的释放，青草型与花香型香气比值降低，从而有利于果实品质的改善。LOX活性受到乙烯的抑制而降低，而ADH和AAT则受乙烯的诱导活性升高，相应的6-C醛类物质在外源乙烯处理的桃果实中含量降低。

关键词: 桃果实, 低温贮藏, 香气, 外源乙烯

Abstract:

In order to explore the regulation of exogenous ethylene on biosynthesis of aroma volatiles in peach during cold (0 ℃) storage, the release of aroma components as well as the changes of LOX, ADH and AAT activity closely related biosynthesis of aroma volatiles were investigated. The peaches stored at ambient temperature and 0 ℃ respectively served as the control 1 and control 2. The results showed that the syntheses of 19 components were inhibited by low temperature and their contents decreased. However the all components, except for menthol, were induced by exogenous ethylene and increased. The other 10 components were induced by low-temperature and increased in the cold storage. Four components of the 10 were induced by exogenous ethylene simultaneously, while the rest six were inhibited by exogenous ethylene. Low temperature repressed the synthesis of menthol, thus it was detected only under room temperature condition. Low-temperature storage resulted in obstacles of floral aroma esters metabolism, and lowered the fruit quality, while exogenous ethylene could promote the biosynthesis of the characteristic aroma linalool and γ-decalactone, inhibit hexanal, trans-2-hexenoic aldehyde and benzaldehyde release, and reduced the radio of the grassy flavor and floral flavor, so that the fruit quality was improved by exogenous ethylene. LOX activity was inhibited by exogenous ethylene, but the activity of ADH and AAT were elevated by exogenous ethylene, leading to reduction of the content of 6-C aldehydes accordingly.

Key words: peach fruit, low temperature storage, aroma volatiles, exogenous ethylene

中图分类号:

S718.43

王贵章, 李杨昕, 王贵禧, 梁丽松, 马庆华. 外源乙烯对冷藏桃果实香气物质合成的调控[J]. 林业科学, 2014, 50(3): 55-62.

Wang Guizhang, Li Yangxin, Wang Guixi, Liang Lisong, Ma Qinghua. Aroma Volatiles Biosynthesis and Relative Enzyme Activities Regulated by Exogenous Ethylene in Peach Fruits Stored at Low Temperature[J]. Scientia Silvae Sinicae, 2014, 50(3): 55-62.

参考文献

胡花丽, 梁丽松, 王贵禧, 等. 2007. 外源乙烯对CA贮藏桃果实MDA含量、PPO和LOX活性变化的影响. 西北林学院学报, 22 (3) : 38- 42.
(Hu H L, Liang L S, Wang G X, et al. 2007. Effect of exogenous ethylene on MDA content,PPO and LOX activities of peach fruit during CA storage. Journal of Northwest Forestry University, 22 (3) : 38- 42. [in Chinese] )
贾惠娟. 2007. 水果香气物质研究进展. 福建果树, (2): 31- 34.
李杨昕, 王贵禧, 梁丽松. 2011. ‘大久保’桃常温贮藏过程中香气成分变化及其与乙烯释放的关系. 园艺学报, 38 (1): 35- 42.
(Li Y X, Wang G X, Liang L S. 2011. The changes of volatile compositions of 'Okubo ' peach and its relationship with ethylene and relevant enzymes stored at ambient temperature. Acta Horticulturae Sinica, 38 (1): 35- 42. [in Chinese] )
茅春林,张上隆. 1999. 采后桃果实中多胺和乙烯对低温胁迫的反应.园艺学报, 26(6) : 360-363.
(Mao L C, Zhang S L. 1999. Responses of polyamines and ethylene in peaches to chilling stress. Acta Horticulturae Sinica, 26(6) : 360-363. [in Chinese] )
王友升, 王贵禧, 梁丽松. 2006. 不同气体成分贮藏对'大久保'桃果实品质的影响. 农业工程学报, 22(8): 214 -218.
(Wang Y S, Wang G X, Liang L S. 2006. Effects of various O_2 and CO_2 concentrations on the quality of "Okubao" peach fruit during storage. Transactions of the Chinese Society of Agricultural Engineering, 22(8): 214 -218. [in Chinese] )
谢永红, 刘国杰, 欧毅, 等. 2006. 温度和GA₃对桃果实采后乙烯合成及桃ETR1同源基因表达的影响. 果树学报, 23 (4): 523-526.
(Xie Y H, Liu G J, Ou Y, et al. 2006. Effects of temperature and GA3 on ethylene biosynthesis and expression of homologous gene APETR1 in post-harvested peaches. Journal of Fruit Science, 23 (4): 523-526. [in Chinese] )
于建娜, 刘文杰. 2006. 库尔勒香梨果实香气成分的气象色谱-质谱分析. 塔里木大学学报, 18(4): 10-12.
(Yu J N, Liu W J. 2006. Analysis of aroma components of Pyrus sinkiangensis yü by gas chromatography-mass spectrometry. Journal of Tarim University, 18(4): 10-12. [in Chinese] )
中华人民共和国农业部.2002.NY/T 586—2002, 鲜桃. 北京:中国标准出版社.
Baldwin E A, Scott J W, Shewmaker C K. 2000. Flavor trivia and tomato aroma: Biochemistry and possible mechanisms for important aroma components. Hortscience, 35 (6): 1021-1031.
Beekwilder J, Alvarez-Huerta M, Neef E, et al. 2004. Functional characterization of enzymes forming volatile esters from straw-berry and banana. Plant Physiology, 135(4): 1865-1878.
Cai C, Xu C J, Shan L L, et al. 2006. Low temperature conditioning reduces postharvest chilling injury in loquat fruit. Postharvest Biology and Technology, 41(3): 252-259.
Cantín C M, Crisosto C H, Ogundiwin E A, et al. 2010. Chilling injury susceptibility in an intra-specific peach \ progeny. Postharvest Biology and Technology, 58(2): 79-87.
Defilippi B G, Kader A A, Dandekar A M. 2005. Apple aroma: alcohol acyltransferase, a rate limiting step for ester biosynthesis, is regulated by ethylene. Plant Science, 168 (5): 1199- 1210.
Dixon J, Hewett E W. 2000. Factors affecting apple Aroma/flavor volatile concentration:review. Journal of Crop and Horticultural Science, 28(3):155-173.
Eduardo I, Chietera G, Bassi D, et al. 2010. Identification of key odor volatile compounds in the essential oil of nine peach accessions. Journal of the Science of Food and Agriculture, 90(7): 1146- 1154.
González-Aguilar G A, Tiznado-Hernández M E, Zavaleta-Gatica R, et al. 2004. Methyl jasmonate treatments reduce chilling injury and activate the defense response of guava fruits. Biochemical and Biophysical Research Communications, 313(3): 694-701.
Hayama H, Shimada T, Fujii H, et al. 2006. Ethylene-regulation of fruit softening and softening-related genes in peach. Journal of Experimental Botany, 57(15): 4071-4077.
Horvat R J, Chapman G W, Robertson J A. 1990. Comparison of the volatile compounds from several commercial peach cultivars. Journal of Agriculture and Food Chemistry, 38(1): 234 -237.
Knee M. 2002. Fruit quality and its biological basis. Sheffield: Sheffield Academic Press.
Leffingwell J C, Leffingwell D. 1991. GRAS flavor chemicals-detection thresholds. Perfumer & Flavorist, 16(1): 2-19.
Longhurst T J, Tung J F, Brady C J. 1994. Development regulation of the expression of alcohol dehydrogenase in ripening tomato fruit. Journal of Food Biochemistry, 14(6): 421- 433.
Lurie S, Crisosto C H. 2005. Chilling injury in peach and nectarine. Postharvest Biology and Technology, 37(3): 195-208.
Pérez A G, Olías R, Luaces P, et al. 2002. Biosynthesis of strawberry aroma compounds through amino acid metabolism. Journal of Agriculture and Food Chemistry, 50(14): 4037- 4042.
Pérez A G, Sanz C, Olias R. 1996. Evolution of strawberry alcohol acyltransferase activity during fruit development and storage. Journal of Agriculture and Food Chemistry, 44(10): 3286- 3290.
Riu-Aumatell M, Castellari M, López-Tamames E, et al. 2004. Characterization of volatile compounds of fruit juices and nectars by HS/SPME and GC/MS. Food Chemistry, 87(4): 627-637.
Wang Y, Yang C, Li S, et al. 2009. Volatile characteristics of 50 peaches and nectarines evaluated by HP-SPME with GC-MS. Food Chemistry, 116 (1): 356-364.
Wang Y S, Tian S P, Xu Y. 2005. Effects of high oxygen concentration on pro- and anti-oxidant enzymes in peach fruits during postharvest periods. Food Chemistry, 91(1): 99-104.
Woolf A B, Cox K A, White A. 2003. Low temperature conditioning treatments reduce external chilling injury of 'Hass' avocados. Postharvest Biology and Technology, 28(1): 113-122.
Yahia E M. 1994. Apple flavor. Horticultural Reviews,16:197-234.
Zhang B, Shen J Y, Wei W W, et al. 2010. Expression of genes associated with aroma formation derived from the fatty acid pathway during peach fruit ripening. Journal of Agriculture and Food Chemistry, 58(10): 6157- 6165.
Zhang B, Yin X R, Li X, et al. 2009. Lipoxygenase gene expression in ripening kiwifruit in relation to ethylene and aroma production. Journal of Agriculture and Food Chemistry, 57(7): 2875-2881.
Zhang X M, Jia H J. 2005. Changes in aroma volatile compounds and ethylene production during ""Hujingmilu"" peach (Prunus persica L.) fruit development. Journal of Plant Physiology and Molecular Biology, 31 (1): 41- 46.
Zhou H W, Dong L, Ben-Arie R, et al. 2001. The role of ethylene in the prevention of chilling injury in nectarines. Journal of Plant Physiology, 158(1): 55- 61.

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外源乙烯对冷藏桃果实香气物质合成的调控

Aroma Volatiles Biosynthesis and Relative Enzyme Activities Regulated by Exogenous Ethylene in Peach Fruits Stored at Low Temperature

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