基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响

doi:10.11707/j.1001-7488.20140307

摘要/Abstract

摘要：

以鹿角杜鹃当年生木质化枝条为材料，采用基质、激素种类及其浓度三因素析因设计，研究各因素及其交互作用对鹿角杜鹃扦插育苗的影响，并运用隶属函数法对各处理组合的育苗效果进行综合评价。结果表明：3个主因素中，激素种类对育苗效果影响最大，激素浓度其次，两者对绝大部分育苗指标的影响达到显著水平；扦插基质影响相对较小，仅对愈伤率、生根率及扦插苗地上部分生长指标有显著影响。两因素交互效应中，激素种类×浓度的交互效应最大，对除新梢数、新梢长外的所有指标有极显著影响；基质×激素浓度互作主要显著影响插穗生根指标；基质×激素种类效应最小，仅对愈伤率、新梢率有显著影响。三因素交互效应对部分生根指标也有显著影响。隶属函数综合评价表明：腐殖土+河沙（3：1）基质与50 mg·L^-1 GA₃组合的隶属值最大，该处理在所有组合中生根率（78.33%）、老叶留存率（90.00%）最高，腐烂率（8.33%）最低，其他育苗指标也有良好的表现，为鹿角杜鹃扦插育苗的最佳组合。

关键词: 鹿角杜鹃, 扦插繁殖, 基质, 激素, 育苗指标

Abstract:

Cuttings of annual woody branches of Rhododendron latoucheae were treated with different hormone types and concentrations in different growing media and the influences of the three factors and their interaction on the cutting propagation were studied using factorial design. The propagation effect of each factor combination was comprehensively evaluated by the subordinate function method. The results showed that, the hormone types had the greatest influence on cutting propagation among the three main factors, followed by hormone concentrations, and the two factors had significant influences on the most cutting propagation parameters. Media had the least influence on cutting propagation, and it nevertheless had significant influence on callusing percentage, rooting percentage and above-ground growth parameter. The analysis results of the interaction of the three factors revealed that, the interaction of hormone types and the concentrations had the biggest effects on cutting propagation, and it brought about most significant difference on all parameters except the number and the length of new shoot. The interaction of media and hormone types had significant influence on rooting parameters. The interaction of media and hormone concentration was least, with significant influence only on callusing percentage and new shoot percentage. The interaction of media, hormone types and concentrations also had significant influences on several rooting parameters. The results of comprehensive evaluation using the subordinate function method showed that the combination of soil+sand (3:1) medium and GA₃(50 mg·L^-1) had the biggest value of subordinate function, and it led to the highest rooting percentage (78.33%) and retention percentage of old leaves (90.00%), and the lowest rotting percentage (8.33%). The other propagation parameters also well performed with the combination in comparison with all other combinations. Thus, this combination should be best for cutting propagation of Rh. latoucheae.

Key words: Rhododendron latoucheae, cutting propagation, media, hormones, propagation parameters

中图分类号:

S723.132

张乐华, 王书胜, 单文, 李晓花, 王凯红, 王兆宏. 基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J]. 林业科学, 2014, 50(3): 45-54.

Zhang Lehua, Wang Shusheng, Shan Wen, Li Xiaohua, Wang Kaihong, Wang Zhaohong. Influences of Growth Media, and Hormone Types and Concentrations on Cutting Propagation of Rhododendron latoucheae[J]. Scientia Silvae Sinicae, 2014, 50(3): 45-54.

参考文献

高俊香, 梅盛龙, 鲁小珍, 等. 2009. 凤阳山自然保护区麂角杜鹃种群结构与分布. 南京林业大学学报: 自然科学版, 33(2): 35-38.
(Gao J X, Mei S L, Lu X Z, et al. 2009. The population structure and distribution of Rhododendron iatoucheae in Fengyangshan Nature Reserve. Journal of Nanjing Forestry University(Natural Sciences Edition), 33(2): 35-38. [in Chinese] )
耿芳, 张冬林, 李志辉, 等. 2008. IBA生根剂对卡罗来纳杜鹃插条生根的影响. 华中农业大学学报, 27(1): 127-130.
(Geng F, Zhang D L, Li Z H, et al. 2008. The influence of IBA rooting hormone on the rooting of Rhododendron carolinianum Rehd.stem cuttings. Journal of Huazhong Agricultural University, 27(1): 127-130. [in Chinese] )
李朝婵, 赵云龙, 张冬林, 等. 2012. 长蕊杜鹃扦插内源激素变化及解剖结构观察. 林业科学研究, 25(3): 360-365.
(Li C C, Zhao Y L, Zhang D L, et al. 2012. Changes of endogenous hormones and anatomical structure of Rhododendron stamineum during cutting propagation. Forest Research, 25(3): 360-365. [in Chinese] )
王凯红, 凌家慧, 张乐华, 等. 2011. 两种常绿杜鹃亚属幼苗耐热性的主成分及隶属函数分析. 热带亚热带植物学报, 19(5): 412-418.
(Wang K H, Ling J H, Zhang L H, et al. 2011. Analysis of principal components and subordinate function on heat tolerance of two seedlings of Rhododendron subgenus hymenanthes. Journal of Tropical and Subtropical Botany, 19(5): 412-418. [in Chinese] )
张长芹, 高连明, 薛润光, 等. 2004. 中国杜鹃花的保育现状和展望. 广西科学, 11(4): 354-359, 362.
(Zhang C Q, Gao L M, Xue R G, et al. 2004. A general review of the research and conservation statue of Chinese Rhododendron. Guangxi Sciences, 11(4): 354-359, 362. [in Chinese] )
张乐华, 刘向平, 王凯红, 等. 2006. 杜鹃属植物种子育苗研究. 园艺学报, 33(6): 1361-1364.
(Zhang L H, Liu X P, Wang K H, et al. 2006. A study on seed germination and seedling growth of Rhododendrons. Acta Horticulturae Sinica, 33(6): 1361-1364. [in Chinese] )
赵云龙, 陈训, 李朝婵. 2013. 糙叶杜鹃扦插生根过程中生理生化分析. 林业科学, 49(6): 45-51.
(Zhao Y L, Chen X, Li C C. 2013. Dynamic of physiology and biochemistry during wild Rhododendron scabrifolium cutting propagation. Scientia Silvae Sinicae, 49(6): 45-51. [in Chinese] )
Abd El-Aal A M K, Shaaban M M. 2011. Why some grapevine cultivars are hard to root?. Australian Journal of Basic and Applied Sciences, 5(2): 110-116.
Afzal M, Khan M A, Pervez M A, et al. 2011. Root induction in the aerial offshoots of date palm (Phoenix dactylifera L.) cultivar, Hillawi. Pakistan Journal of Agricultural Sciences, 48(1): 11-17.
Ammarellou A, Kazemeitabar K, Najafei H Z, et al. 2012. Effects of different culture media on rooting of Urtica dioica L. stem cuttings. Journal of Soil Science and Environmental Managemen, 3(7): 172-175.
Bartel B, LeClere S, Magidin M, et al. 2001. Inputs to the active indole-3-acetic acid pool: de novo synthesis, conjugate hydrolysis, and indole-3-butyric acid β-oxidation. Journal of Plant Growth Regulation, 20(3): 198-216.
Bashir M A, Anjum M A, Chaudhry Z, et al. 2009. Response of jojoba (Simmondsia chinensis) cuttings to various concentrations of auxins. Pakistan Journal of Botany, 41(6): 2831-2840.
Chen X J, Zhou Y, Zhang Q X, et al. 2009. Cutting propagation of wild Rhododendron decorum Franch. HortScience, 44(4): 1104.
Dolor D E, Ikie F O, Nnaji G U. 2009. Effect of propagation media on the rooting of leafy stem cuttings of Irvingia wombolu (Vermoesen). Research Journal of Agriculture and Biological Sciences, 5(6): 1146-1152.
Ernstsen A, Hansen J. 1986. Influence of gibberellic acid and stock plant irradiance on carbohydrate content and rooting in cuttings of Scots pine seedlings (Pinus sylvestris L.). Tree Physiology, 1(1): 115-125.
Felippe G M. 1980. Bud breaking and adventitious root formation in Panicum maximum Jacq. Biologia plantarum, 22(5): 392-395.
Ferriani A P, Bortolini M F, Zuffellato-Ribas K C, et al. 2006. Vegetative propagation by cuttings of azaléia tree (Rhododendron thomsonii HOOK. f.). Semina: Ciências Agrárias, 27(1): 35-42.
Gensel W H, Blazich F A. 1985. Propagation of Rhododendron chapmanii by stem cuttings. Journal of Environmental Horticulture, 3(2): 65-68.
Gou J Q, Strauss S H, Tsai C J, et al. 2010. Gibberellins regulate lateral root formation in Populus through interactions with auxin and other hormones. Plant Cell, 22(3): 623-639.
Husen A. 2012. Changes of soluble sugars and enzymatic activities during adventitious rooting in cuttings of Grewia optiva as affected by age of donor plants and auxin treatments. American Journal of Plant Physiology, 7(1): 1-16.
Kochhar S, Singh S P, Kochhar V K. 2008. Effect of auxins and associated biochemical changes during clonal propagation of the biofuel plant—Jatropha curcas. Biomass Bioenergy, 32(12): 1136-1143.
Laubscher C P, Ndakidemi P A. 2008. Rooting success using IBA auxin on endangered Leucadendron laxum (PROTEACEAE) in different rooting mediums. African Journal of Biotechnology, 7(19): 3437-3442.
Lü J F, Silva J A T, Ma G H. 2012. Vegetative propagation of Primulina tabucum Hance by petiole cuttings. Scientia Horticulturae, 134(1): 163-166.
Mohammadi A T, Vahid S. 2012. Rooting in hibiscus rosa-sinensis (yellow double hybrid') by indole butyric acid and rooting substrates. International Journal of Plant, Animal and Environmental Sciences, 2(2): 194-197.
Nanda K K, Anand V K, Chibbar R N. 1972. The promotive effect of gibberellic acid on the production of adventitious roots on stem cuttings of Ipomoea fistulosa. Planta, 105(4): 360-363.
Nawrocka-Grze?kowiak U. 2004. Effect of growth substances on the rooting of cuttings of Rhododendron species. Folia Horticulturae, 16(1): 115-123.
Niu S H, Li Z X, Yuan H W, et al. 2013. Proper gibberellin localization in vascular tissue is required to regulate adventitious root development in tobacco. Journal of Experimental Botany, 64(11): 3411-3424.
Nordström A C, Jacobs F A, Eliasson L. 1991. Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings. Plant Physiology, 96(3): 856-861.
Rana R S, Sood K K. 2012. Effect of cutting diameter and hormonal application on the propagation of Ficus roxburghii Wall. through branch cuttings. Annals of Forest Research, 55(1): 69-84.
Shah M, Khattak A M, Amin N. 2006. Effect of different growing media on the rooting of Ficus binnendijkii 'Amstel Queen' cuttings. Journal of Agricultural and Biological Science, 1(3): 15-17.
Singh, K K, Gurung B. 2009. In vitro propagation of R. maddeni Hook. F. an endangered rhododendron species of Sikkim Himalaya. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37(1): 79-83.
Singh K K, Gurung B, Rai L K, et al. 2010. The influence of temperature, light and pre-treatment on the seed germination of critically endangered Sikkim Himalayan Rhododendron (R. niveum Hook f.). Journal of American Science, 6(8): 172-177.
Strzelecka K. 2007. Anatomical structure and adventitious root formation in Rhododendron ponticum L. cuttings. Acta Scientiarum Polonorum, 6 (2): 15-22.
Suleiman M K,Bhat N R, Jacob S, et al. 2011. Effect of growth regulators on rooting of hardwood cuttings of Lycium shawii, Nitraria retusa and Farsetia aegyptia. Journal of Agricultural Science and Technology B, 1(3): 414-419.
Swamy S L, Puri S, Singh A K. 2002. Effect of auxins (IBA and NAA) and season on rooting of juvenile and mature hardwood cuttings of Robinia pseudoacacia and Grewia optiva. New Forests, 23(2): 143-157.

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