关键词: 文心兰, 试管苗, 树脂膜培养容器, 低温贮藏

Abstract:

The goal of this study was to evaluate the effect of storage containers and manners, pretreatment time in low temperature on Oncidium variety ‘Aloha Iwanaga’ plantlets in vitro. Results showed that the polymer film culture bag (CB) was an effective container for Oncidium plantlets in vitro, and the CB with liquid medium (CB·L) was also a more economical and effective one for plantlets in vitro among the methods of CB·L, the flask with liquid medium (FL·L) and the flask with solid medium (FL·S) during low temperature storage. The comprehensive index, including survival rate after storage and rate of normal plantlet and axillary plantlet formation after further culture, of plantlets in vitro with the treatments of pretreatment time of 5 days or 7 days in the CB·L method was higher than other methods among the five pretreatment times of 0 day (the plantlet was stored immediately after inoculation), 3 days, 5 days, 7days and 9 days. The physiological indexes, as plantlet height, leaf number, number and length of root and dry mass rate, of plantlets in vitro in the CB·L method were a little higher than others and however no significantly different except the fresh/dry mass. In conclusion, the suitable pretreatment time for Oncidium plantlets in vitro was 5 to 7 days.

Key words: Oncidium, plantlet in vitro, polymer film culture bag (CB), low temperature storage