毛白杨和烟草次生维管发育相关NAC转录因子的比较

doi:10.11707/j.1001-7488.20140304

摘要/Abstract

摘要： 烟草具有典型的次生维管发育过程，与毛白杨在转录组水平上进行比较，有利于通过烟草来鉴定出次生维管发育相关的关键基因。通过高通量测序技术获得烟草维管组织的转录组数据，将NAC转录因子基因通过BLASTP程序比对毛果杨基因组数据库，共得到43对NAC直系同源基因，并根据序列同源性将其分为19个亚组。利用烟草和毛白杨的基因表达谱，分析NAC基因表达量变化，找到13对表达模式相似的烟草和毛白杨的同源基因。选取8对NAC同源基因通过相对荧光定量PCR进行不同组织的表达分析，结果表明这8对NAC同源基因在烟草和毛白杨中的表达模式非常相似。研究结果为利用烟草病毒诱导的基因沉默（VIGS）技术研究NAC转录因子，进而揭示杨树的维管发育相应基因的功能提供基础。

关键词: 烟草, 杨树, NAC, 维管发育

Abstract: Tobacco (Nicotiana tabacum) plants have typical secondary vascular development process. It is useful for identifying key genes in the secondary vascular development based on the transcriptome comparison between tobacco and Populus tomentosa. Totally 43 pairs of NAC orthologous genes were identified using BLASTP search in the entire Populus trichocarpa genome sequence with NAC transcription factor genes identified in tobacco vascular tissue transcriptome database as probe and were divided into 19 subgroup based on the sequence homology. The analyses of gene expression levels obtained using RNA-seq in tobacco and P. tomentosa suggest that there are 13 pairs of NAC orthologous genes sharing similar expression patterns in tobacco and P. tomentosa. Eight pairs of NAC orthologous genes were selected to perform expression analysis using relative real time RT-PCR in different tissues of tobacco and P. tomentosa. The results indicated that the 8 pairs of NAC orthologous genes shared similar expression patterns in tobacco and P. tomentosa. It is suggested feasibility to study the functions of corresponding poplar genes through studying functions of tobacco NAC transcription factor using tobacco VIGS system and the study provides the basic data for studying the vascular tissue development regulation.

Key words: Nicotiana tabacum, Populus tomentosa, NAC, vascular development

中图分类号:

S718.46

郭伟, 卢孟柱. 毛白杨和烟草次生维管发育相关NAC转录因子的比较[J]. 林业科学, 2014, 50(3): 22-30.

Guo Wei, Lu Mengzhu. Comparison of NAC Transcription Factor Involved in Secondary Vascular Development between Populus tomentosa and Tobacco[J]. Scientia Silvae Sinicae, 2014, 50(3): 22-30.

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