关键词: 黄檗, AM真菌, PCR-DGGE, 菌群结构, 多样性

Abstract:

In this study, the mycorrhizae of Phellodendron amurense in three different habitats were used as the research objects. The root samples were investigated by means of acid fuchsin stain after they were lysed in alkaline solution. Then, the community composition and diversity of different AM fungi species in samples were analyzed using PCR-DGGE. DGGE results were analyzed in conjunction with DGGE profiles,DNA sequencing and phylogenetics to investigate the pattern of dynamic variation of the AM fungiof Phellodendron amurense in three different habitats, lay the foundation for the study for functional flora in mycorrhizal of P.amurense. The results showed that the roots and AM fungi of P.amurense in different habitats were able to form symbiosis. Distinct differences were detected in infection rate, DGGE band abundance and dominancy. The infection rate, abundance, dominancy and diversity index of the mycorrhizae in urban plantation areas were significantly higher than that in natural secondary forest and natural primary forest. All the detection indexes of the mycorrhizae in natural primary forest was lowest. Sequencing and phylogenetic analysis revealed these sequences could be divided in four groups: Glomus, Scutellospora, Diversispora and Hyponectria buxi. Glomus fungi were the dominant species in the roots of P.amurense in the urban plantation, natural secondary forest and natural primary forest.

Key words: Phellodendron amurense, AM fungi, PCR-DGGE, community composition, diversity