多杀菌素对舞毒蛾幼虫解毒酶活性的影响

doi:10.11707/j.1001-7488.20120913

林业科学 ›› 2012, Vol. 48 ›› Issue (9): 82-87.doi: 10.11707/j.1001-7488.20120913

多杀菌素对舞毒蛾幼虫解毒酶活性的影响

鄢杰明, 钟华, 严俊鑫, 严善春

东北林业大学林木遗传育种国家重点实验室哈尔滨 150040

收稿日期:2011-01-19 修回日期:2012-02-17 出版日期:2012-09-25 发布日期:2012-09-25
通讯作者: 严善春

Effect of Spinosad to Detoxifying Enzymes Activity in Lymantria dispar Larva

Yan Jieming, Zhong Hua, Yan Junxin, Yan Shanchun

State Key Laboratory of Forest Genetics and Tree Breeding Northeast Forestry University Harbin 150040

Received:2011-01-19 Revised:2012-02-17 Online:2012-09-25 Published:2012-09-25

摘要/Abstract

摘要：

为了研究多杀菌素对森林害虫舞毒蛾的杀虫活性,采用叶片药膜法测定多杀菌素对舞毒蛾3龄和5龄幼虫的生物活性及致死中量对其体内解毒酶活性的影响。结果表明:多杀菌素24 h致死中浓度(LC₅₀)分别为0.024 9 mg·L^-1(3龄)和0.226 9 mg·L^-1(5龄);致死中量多杀菌素对舞毒蛾3龄和5龄幼虫体内解毒酶存在不同程度的影响,其中对羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GST)、多功能氧化酶(MFO)和碱性磷酸酯酶(ALP)大体上均表现为先激活后抑制作用,差异显著(P<0.05);而对酸性磷酸酯酶表现为抑制-激活-抑制作用,差异极显著(P<0.01)。LC₁₀和LC₅₀剂量的多杀菌素对舞毒蛾3龄幼虫的发育历期无显著影响,但能显著降低幼虫成活率及化蛹率(P<0.05)。多杀菌素对舞毒蛾有较强杀虫活性,能够有效影响舞毒蛾的生化指标,干扰其正常的生长发育,可以作为防治舞毒蛾的安全有效药剂。

关键词: 多杀菌素, 舞毒蛾, 解毒酶

Abstract:

In order to study insecticidal activity of spinosad against Lymantria dispar, bioactivity and LC₅₀ of spinosad against the 3rd and 5th instar larva of L. dispar and its effect on activities of the detoxifying enzymes were assayed in this study. The results showed that LC₅₀ of spinosad in 24 h was 0.0249 mg·L^-1 against 3rd instar larva and 0.2269mg ·L^-1 against 5th instar larva. Spinosad had various effects on activities of the detoxifying enzymes under the LC₅₀ treatment. Carboxylesterase (CarE), glutathione-S-transferase (GST), multi-function oxidase (MFO) and alkaline phosphatase (ALP) were firstly significantly increased and then decreased (P <0.05); But for acid phosphatase (ACP), the change pattern in activity were decreased-increased-decreased, and the variation was significant (P< 0.01). The development duration of the larva treated with LC₁₀ and LC₅₀ of spinosad was not significantly different from control, but the larval survival rate and pupation rate were significantly lower than control (P<0.05). The result indicated that the biochemical indexes of L. dispar were affected by spinosad. Therefore, spinosad is a safe and effective pesticide for L. dispar.

Key words: spinosad, Lymantria dispar, detoxifying enzymes

中图分类号:

S763.306

鄢杰明;钟华;严俊鑫;严善春. 多杀菌素对舞毒蛾幼虫解毒酶活性的影响[J]. 林业科学, 2012, 48(9): 82-87.

Yan Jieming;Zhong Hua;Yan Junxin;Yan Shanchun. Effect of Spinosad to Detoxifying Enzymes Activity in Lymantria dispar Larva[J]. Scientia Silvae Sinicae, 2012, 48(9): 82-87.

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多杀菌素对舞毒蛾幼虫解毒酶活性的影响

Effect of Spinosad to Detoxifying Enzymes Activity in Lymantria dispar Larva

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