杜鹃红山茶花芽分化及其代谢产物的变化

doi:10.11707/j.1001-7488.20120813

林业科学 ›› 2012, Vol. 48 ›› Issue (8): 81-86.doi: 10.11707/j.1001-7488.20120813

杜鹃红山茶花芽分化及其代谢产物的变化

李辛雷^1,2, 孙振元², 李纪元¹, 范正琪¹, 殷恒福¹

1. 中国林业科学研究院亚热带林业研究所富阳 311400;2. 林木遗传育种国家重点实验室中国林业科学研究院林业研究所北京 100091

收稿日期:2012-01-31 修回日期:2012-04-16 出版日期:2012-08-25 发布日期:2012-08-25
通讯作者: 孙振元

Changes of Metabolites and Flower Bud Differentiation of Camellia azalea

Li Xinlei^1,2, Sun Zhenyuan², Li Jiyuan¹, Fan Zhengqi¹, Yin Hengfu¹

1. Research Institute of Subtropical Forestry, CAF Fuyang 311400;2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, CAF Beijing 100091

Received:2012-01-31 Revised:2012-04-16 Online:2012-08-25 Published:2012-08-25

摘要/Abstract

摘要：

以杜鹃红山茶为材料,采用石蜡切片法观察花芽分化过程,研究该过程与外部形态的相关性及其代谢产物的变化。结果表明:杜鹃红山茶花芽分化于5—9月间持续不断进行,该时段内能观察到处于不同分化阶段的花芽; 其过程可分为生理分化期、花原基分化期、萼片原基分化期、花瓣原基分化期、雄蕊原基分化期、雌蕊原基分化期。杜鹃红山茶花芽分化过程与其外部形态特征之间有着相对稳定的关系,可以通过花芽形态特征来对其进行判别。花芽分化期可溶性蛋白质含量先升高后降低,可溶性糖含量及可溶性糖/可溶性蛋白质先降低后升高; RNA、总核酸含量及RNA/DNA的变化趋势一致,均随花芽分化逐渐升高,在花瓣原基分化期达到最高,雄蕊、雌蕊原基分化期降低,而DNA 含量在整个过程中一直处于较低水平且变化平缓。

关键词: 杜鹃红山茶, 花芽, 分化, 代谢物质

Abstract:

The paraffin section method was used to observe the process of flower bud differentiation of Camellia azalea. Metabolites in the process and the correlation between flower bud differentiation and its external morphology were studied. The results showed that the flower bud differentiation of C. azalea progressed continuously from May to September, during which the flower buds in different differentiation phases could be observed at anytime point. The process of flower bud differentiation could be divided into six phases: physiological differentiation, flower primordium differentiation, sepal primordium differentiation, petal primordium differentiation, stamen and pistil primordium differentiation phases. The process could be identified by the external morphology according to the stable relations between the differential phases and the corresponding morphological characteristics. The content of soluble protein in the flower bud increased at first and then decreased, and soluble sugar content and the ratio of soluble sugar and soluble protein were opposite during the flower bud differentiation. The changes in RNA and total content of nucleic acid were similar to the change of RNA/DNA, which gradually increased with flower bud differentiation and reached a peak in the phase of petal primordium differentiation, then gradually declined in the phase of stamen and pistil primordium differentiation, while DNA content was low and changed gently in the process of flower bud differentiation.

Key words: Camellia azalea, flower bud, differentiation, metabolites

中图分类号:

S718.43

李辛雷;孙振元;李纪元;范正琪;殷恒福. 杜鹃红山茶花芽分化及其代谢产物的变化[J]. 林业科学, 2012, 48(8): 81-86.

Li Xinlei;Sun Zhenyuan;Li Jiyuan;Fan Zhengqi;Yin Hengfu. Changes of Metabolites and Flower Bud Differentiation of Camellia azalea[J]. Scientia Silvae Sinicae, 2012, 48(8): 81-86.

[1]	张守科, 方林鑫, 刘亚宁, 王毅, 张威, 舒金平, 张亚波, 汪阳东, 王浩杰. 茶籽象ATP合成酶基因在不同海拔选择压力下的遗传分化及结构变异[J]. 林业科学, 2019, 55(6): 65-73.
[2]	尹淑艳, 李波, 周成刚, 张卫光, 谢丽霞, 刘永杰. 基于28S rDNA分析板栗和杉木上针叶小爪螨物种分化原因[J]. 林业科学, 2019, 55(4): 122-128.
[3]	孙芳芳, 聂迎彬, 马松梅, 魏博, 吉万全. 基于ITS和cpDNA序列的梭梭和白梭梭物种分化[J]. 林业科学, 2019, 55(3): 43-53.
[4]	王婧如, 王明浩, 张晓玮, 孙杉, 赵长明. 同倍体杂交物种紫果云杉的生态位分化及其未来潜在分布区预测[J]. 林业科学, 2018, 54(6): 63-72.
[5]	孙菲, 杨春红, 李波, 郑金柱, 周成刚, 谢丽霞, 尹淑艳. 针叶小爪螨不同种群内共生菌Wolbachia和Cardinium的检测及系统发育分析[J]. 林业科学, 2017, 53(8): 141-148.
[6]	杨盛, 郝国伟, 张晓伟, 白牡丹, 李凯, 石美娟, 程培红, 郭黄萍, 李六林. ‘玉露香梨’僵芽发生与矿质营养的关系[J]. 林业科学, 2016, 52(2): 127-133.
[7]	刘霞, 徐正会, 于娜娜, 张成林. 藏东南嘎隆拉山及墨脱河谷蚂蚁物种的分布格局[J]. 林业科学, 2016, 52(11): 88-95.
[8]	王雁红, 俞琦, 杨佳, 赵鹏, 李忠虎, 赵桂仿. 基于核微卫星的短柄枹栎居群遗传多样性和遗传结构[J]. 林业科学, 2015, 51(12): 121-131.
[9]	杜玉梁, 刘彩虹, 陈叶, 尹增芳. 管状分子分化离体培养系统的建立及影响因素[J]. 林业科学, 2014, 50(8): 146-153.
[10]	孙颖, 谭晓风, 罗敏, 李建安. 油桐花芽2个不同发育时期转录组分析[J]. 林业科学, 2014, 50(5): 70-74.
[11]	刘家霖, 王传宽, 张全智. 不同分化等级兴安落叶松树干心材和边材的空间变异[J]. 林业科学, 2014, 50(12): 114-121.
[12]	杨钤, 谢映平, 樊金华, 邵生富, 吴俊, 王彦士, 赵常胜, 张英伟. 日本松干蚧3个地理种群的遗传分化[J]. 林业科学, 2013, 49(12): 88-96.
[13]	夏明瑞;周国娜;高宝嘉;. 不同林分类型油松毛虫CO Ⅰ 基因变异分析[J]. 林业科学, 2012, 48(9): 171-175.
[14]	尹淑艳;李波;郭慧玲;李会;李杨;孙绪艮. 基于mtDNA-COI基因序列的针叶小爪螨种群遗传结构影响因素分析[J]. 林业科学, 2012, 48(2): 162-168.
[15]	李楠;柳新红;李因刚;李海波;盛炜彤;惠刚盈;郑勇奇. 白花树天然群体的遗传多样性[J]. 林业科学, 2012, 48(11): 49-56.

杜鹃红山茶花芽分化及其代谢产物的变化

Changes of Metabolites and Flower Bud Differentiation of Camellia azalea

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式