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林业科学 ›› 2012, Vol. 48 ›› Issue (1): 181-185.doi: 10.11707/j.1001-7488.20120130

• 研究简报 • 上一篇    下一篇

瑞香狼毒超临界萃取物的化学成分及其杀螨活性

梁为1,2, 成军2,3, 卜春亚2,3, 靳永胜2,3, 师光禄2,3, 王有年2,3   

  1. 1. 北京林业大学 北京 100083;2. 农业部都市农业(北方)重点开放实验室 北京 102206;3. 北京农学院 北京 102206
  • 收稿日期:2010-04-26 修回日期:2010-12-01 出版日期:2012-01-25 发布日期:2012-01-25
  • 通讯作者: 王有年

Componential Analysis and Acaricidal Activities of Stellera chamaejasme Extracts by Supercritical Fluid Extraction

Liang Wei1,2, Cheng Jun2,3, Bu Chunya2,3, Jin Yongsheng2,3, Shi Guanlu2,3, Wang Younian2,3   

  1. 1. Beijing Forestry University Beijing 100083;2. Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture Beijing 102206;3. Beijing University of Agriculture Beijng 102206
  • Received:2010-04-26 Revised:2010-12-01 Online:2012-01-25 Published:2012-01-25

关键词: 瑞香狼毒, 超临界萃取, GC/MS, 朱砂叶螨, 杀螨活性, 角鲨烯

Abstract:

Extracts of Stellera chamaejasme by supercritical fluid extraction (SFE) were shown to have acaricidal activities against Tetranychus cinnabarinus. In this experiment, the supercritical parameters of CO2 in SFE were optimized for extracting the components of Stellera chamaejasme by uniform experimental design, and the extracts were further analyzed by GC/MS method. The results showed that the optimal condition of supercritical CO2 extraction was extracting pressure 49 MPa, extracting temperature 15 ℃, resolution pressure 43 MPa, and resolution temperature 6.25 ℃. Under the optimal condition, the theoretical extract rate was 3.77%, and the measured extract rate was 3.75%. Extracts of Stellera chamaejasme with the optimal SFE condition had higher contact and systemic toxicity against Tetranychus cinnabarinus in comparison with that by cold-soaked extraction method, with LC50 value of 2.407 mg·mL-1 and 2.990 mg·mL-1, respectively. There were 13 compounds detected in the extracts, which accounted for 99.99% of all components. The major compounds were squalene, 9,12-octadecadienoic acid (Z,Z)-,n-hexadecanoic acid,quinoline, and campesterol. Squalene was for the first time found from S. chamaejasme. Squalene was found to have contact and systemic toxicity against T. cinnabarinus, with LC50 value of 9.918 mg·mL-1 and 12.918 mg·mL-1, respectively.

Key words: Stellera chamaejasme, supercritical fluid extraction (SFE), GC/MS, Tetranychus cinnabarinus, acaricidal activities, squalene

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