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林业科学 ›› 2011, Vol. 47 ›› Issue (11): 199-203.doi: 10.11707/j.1001-7488.20111132

• 研究简报 • 上一篇    下一篇

牵牛子杀螨活性物质鉴定及其作用机制初探

杜娟1, 赵磊1, 师光禄1, 王有年1, 仝宝生2   

  1. 1. 农业部都市农业(北方)重点开放实验室 北京农学院生物技术学院 北京102206;2. 内蒙古永业生物技术有限责任公司 呼和浩特 010010
  • 收稿日期:2010-03-30 修回日期:2010-12-13 出版日期:2011-11-25 发布日期:2011-11-25
  • 通讯作者: 师光禄

A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus

Du Juan1, Zhao Lei1, Shi Guanglu1, Wang Younian1, Tong Baosheng2   

  1. 1. Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture ; College of Biotechnology, Beijing University of Agriculture Beijing 102206;2. Inner Mongolia Yongye Biotechnology Co., Ltd. Huhhot 010010
  • Received:2010-03-30 Revised:2010-12-13 Online:2011-11-25 Published:2011-11-25

关键词: 朱砂叶螨, 亚油酸甲酯, 谷胱甘肽-S-转移酶, 乙酰胆碱酯酶, 单胺氧化酶, Ca2+-ATP酶, Na+, K+-ATP酶

Abstract:

Fraction 8 of the extractants from semen pharbitidis was identified and its mechanism of biological activity against Tetranychus cinnabarinus was investigated. The main component of the extractant fraction 8 was detected with nuclear magnetic resonance (NMR), gas chromatography and mass spectrum (GC-MS). Influences of the main component on several enzymes activities such as glutathione-S-transferase (GSTs), acetylcholine esterase (AChE) and mono-amine oxidase (MAO), in T. cinnabarinus body were detected by using biochemical method. Results showed that the main component of the extractant fraction 8 was methyl linoleate. The methyl linoleate activated glutathione-S-transferase in T. cinnabarinus body, while it inhibited to various degrees acetylcholine esterase and mono-amine oxidase related to nervous system, and hence caused the death of T. cinnabarinus. Therefore the methyl linoleate has a good prospect developing into new pesticide.

Key words: Tetranychus cinnabarinus, methyl linoleate, glutathione-S-transferase, acetylcholine esterase, mono-amine oxidase, Ca2+-ATPase, Na+, K+-ATPase

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