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林业科学 ›› 2010, Vol. 46 ›› Issue (10): 91-94.doi: 10.11707/j.1001-7488.20101015

• 论文 • 上一篇    下一篇

大戟狼毒对朱砂叶螨的毒性作用

刘素琪1,顾雅静1,王海香2,董海龙1,师光禄3,曹挥1   

  1. 1.山西农业大学农学院 太谷 030801;2 山西农业大学林学院 太谷 030801;3 北京市农业应用新技术重点实验室 北京 102206
  • 收稿日期:2009-05-07 修回日期:2010-06-02 出版日期:2010-10-25 发布日期:2010-10-25
  • 通讯作者: 曹挥

Acarocidal Actions of Euphorbia fischeriana against Tetranychus cinnabarinus

Liu Suqi1;Gu Yajing1;Wang Haixiang2;Dong Hailong1;Shi Guanglu3;Cao Hui1   

  1. 1 Agronomy College Shanxi Agricultural University Taigu 030801;2 Forest College Shanxi Agricultural University Taigu 030801;3 Key Laboratory of New Technology of Agricultural Application Beijing 102206
  • Received:2009-05-07 Revised:2010-06-02 Online:2010-10-25 Published:2010-10-25

摘要:

研究大戟狼毒石油醚提取物中最佳杀螨活性流分11(EFPE-11)处理雌成螨后的症状表现 并采用生化方法测定EFPE-11对朱砂叶螨体内的蛋白含量、谷胱甘肽-S-转移酶、乙酰胆碱酯酶和Ca2+-Mg2+ ATP酶活性的影响。从症状观察初步推测它们可能通过影响朱砂叶螨的神经传导而导致雌成螨死亡。EFPE-11击倒作用迅速,从12 h开始朱砂叶螨就进入了大量死亡期。在施药过程中,EFPE-11引起成螨体内谷胱甘肽-S-转移酶的活性被激活,而乙酰胆碱酯酶活性则被一定程度的抑制,Ca2+-Mg2+ ATP酶活性最后被强烈抑制。乙酰胆碱酯酶和Ca2+-Mg2+ ATP酶的被抑制,会造成神经传递的阻断,导致螨体的死亡,而神经传递的阻断,可能是EFPE-11作用螨体的主要方式。

关键词: 大戟狼毒, 朱砂叶螨, 作用机制

Abstract:

The fraction 11 (EFPE-11) extract of Euphorbia fischeriana with petroleum ether was tested for bio-activity and mechanisms, including the effect on the protein content, the activities of GSTs, AchE and Ca2+-Mg2+ATPase of Tetranychus cinnabarinus. The symptomatic expression and the results of the action mechanism indicated that EFPE-11 could interrupt the nerve transmission of mites. The survival rate of mites began to decrease quickly when treated with EFPE-11 in 12 h. The activity of GSTs in treated mites was activated, but the activity of acetyl cholinesterase(AchE) was inhibited. The activity of Ca2+-Mg2+ATPase was strongly inhibited. The inhibition of the AchE and Ca2+-Mg2+ATPase activity can cause interruption of the nerve transmission and eventually cause mite death. The results suggested that the interruption of the nerve conduction could be the main reason for EFPE-11 against mites.

Key words: Euphorbia fischeriana, Tetranychus cinnabarinus, action mechanism