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林业科学 ›› 2010, Vol. 46 ›› Issue (6): 43-50.doi: 10.11707/j.1001-7488.20100607

• 论文 • 上一篇    下一篇

植物CBF1转录因子的生物信息学分析

蒋瑶,陈其兵   

  1. 四川农业大学林学院雅安 625014
  • 收稿日期:2009-05-18 修回日期:1900-01-01 出版日期:2010-06-25 发布日期:2010-06-25
  • 通讯作者: 陈其兵

Bioinformatic Analysis of CBF1 Transcription Factors from the Plants

Jiang Yao;Chen Qibing   

  1. <i>Forestry College,Sichuan Agricultural UniversityYa' an</i> 625014
  • Received:2009-05-18 Revised:1900-01-01 Online:2010-06-25 Published:2010-06-25

摘要:

利用生物信息学手段,对GenBank中18种不同植物CBF1的氨基酸序列进行比对及系统发育树的构建,并对其氨基酸的组成、等电点、亲水/疏水性、蛋白质的二级结构和三级结构及功能域进行分析预测。结果表明:18种不同植物CBF1的N端存在富含精氨酸和赖氨酸残基的核定位信号(NLS)、与DNA结合的AP2核心结构域以及C端的酸性氨基酸。构建CBF1进化树,并对其分子进化进行探讨。CBF1的氨基酸主要是脂肪族类,都含有非极性氨基酸Ala,其蛋白等电点多数为酸性,且为不稳定亲水性蛋白。α-螺旋和无规则卷曲是18种不同植物CBF1最大量的结构元件,而β-转角和延伸链则散布于整个蛋白质中。不同植物CBF1蛋白的亚细胞定位存在一定的差异。三维结构检测表明此模型的结构符合立体化学规则。

关键词: CBF1, 生物信息学, 分子进化

Abstract:

Multiple alignment of amino acid sequences of CRT-binding factor1(CBF1) from 18 different plants was conducted and a phylogenetic tree with these proteins was built.In addition,the characters of the amino acid constitutions,isoelectric point,hydrophilic/hydrophobic properties,secondary structures,tertiary structures and functional domains of these proteins were analyzed with the tools of bioinformatics software.The results showed that nuclear localization signals(NLS) were rich in Argnine(Arg) and Lysine(Lys) residues at N-terminal of CBF1 in 18 different plants,while containing AP2 core domains which bind to DNA and acidic amino acids were located in C-terminal.Molecular evolution was analyzed by cladogram construction of CBF1.Amino acids of CBF1 mainly belonged to aliphatic series.All CBF1s in 18 different plants contained non-polar amino acid Ala,and most of the isoelectric points were acid,the proteins of CBF1 were unstable and hydrophilic.Constructions of α-helixs and random coils in CBF1 were dominant elements from 18 different plants,β-turns and extended strains interspersed over the whole proteins.Subcellular localizations of CBF1 protein were different.A three-dimensional structure test validated that the modeling accorded with the stereochemistry.This study provides basic information for the research of gene structure and biological function,and also provides evidence for the analysis of the function of interaction in different signal pathway and regulating mechanism.

Key words: CRT-binding factor, bioinformatics, molecular evolution