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林业科学 ›› 2009, Vol. 12 ›› Issue (12): 95-99.doi: 10.11707/j.1001-7488.20091216

• 论文 • 上一篇    下一篇

4-己基间苯二酚和曲酸对舞毒蛾酚氧化酶的抑制作用*

赵燕1,2 1 曹露凡1 周成刚1,3 罗万春1   

  1. 1.山东农业大学植物保护学院 泰安 271018; 2.济南市林业局 济南 250102; 3.山东省林业有害生物防控研究中心 泰安 271000
  • 收稿日期:2008-03-21 修回日期:1900-01-01 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 周成刚
  • 基金资助:
     

Inhibitory Effects of Tetra-hexylresorcinol and Kojic Acids on the Phenoloxidase from Lymantria dispar

Zhao Yan1,2,Yang Long1,Cao Lufan1,Zhou Chenggang1,3,luo wochun1   

  1. 1.College of Plant Protection, Shandong Agricultural University Tai’an 271018;2.Jinan Forestry Administration Jinan 250102; 3.Research Center of Forest Pest Management of Shandong Province Tai'an 271000
  • Received:2008-03-21 Revised:1900-01-01 Online:2009-12-25 Published:2009-12-25
  • Supported by:
     

摘要:

以舞毒蛾为材料,分离提取酚氧化酶,研究4-己基间苯二酚(4HR)和曲酸对舞毒蛾酚氧化酶活力的影响。结果表明: 4HR对该虫的单酚酶和二酚酶活性均表现很强的抑制作用,其IC50分别为0.000 41和0.000 35 mmol·L-1。其中,4HR对单酚酶活力表达的迟滞时间有明显的延长效应,浓度为0.000 2 mmol·L-1时可使单酚酶活力表达迟滞时间从181 s 延长到253 s; 而当浓度为0.000 5 mmol·L-1时,其迟滞时间则延长至372 s。以邻苯二酚为底物时,4HR对二酚酶的抑制作用表现为典型的竞争型抑制类型,抑制常数KI为0.000 15 mmol·L-1。曲酸对舞毒蛾的单酚酶和二酚酶活性均表现较强的抑制作用,其IC50分别为0.06和0.92 mmol·L-1。曲酸对单酚酶活力表达的迟滞时间也有明显的延长效应,浓度为0.1 mmol·L-1时可使单酚酶活力表达迟滞时间从306 s 延长到732 s; 而当浓度为0.15 mmol·L-1 时,其迟滞时间则延长至900 s。以邻苯二酚为底物时,曲酸对二酚酶的抑制作用表现为典型的竞争型抑制类型,抑制常数KI 为0.51 mmol·L-1

关键词: 舞毒蛾, 酚氧化酶, 抑制机制, 4-己基间苯二酚(4HR), 曲酸

Abstract:

The inhibitory effects of tetra-hexylresorcinol and kojic acids on the phenoloxidase from Lymantria dispar were investigated. The results showed that the IC50 of tetra-hexylresorcinol was 0.000 41 mmol·L-1 for monophenolase activity and 0.000 35 mmol·L-1 for diphenolase, respectively. Tetra-hexylresorcinol extended the lagtime of the enzyme for oxidation of L-tyrosine. The lagtime extended from 181 s to 253 s by 4-hexylresorcinol with 0.000 2 mmol·L-1, and the lagtime extended from 181 s to 372 s by the chemical with 0.000 5 mmol·L-1. The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that 4-hexylresorcinol was a competitive inhibitor for the oxidation of catechol and the inhibition constant was 0.000 15 mmol·L-1.The IC50 of kojic acids were 0.06 mmol·L-1 for monophenolase activity and 0.92 mmol·L-1 for diphenolase, respectively. Kojic acids extended the lagtime of the enzyme for oxidation of L-tyrosine. The lagtime extended from 306 s to 702 s by the kojic acids with 0.1 mmol·L-1, and the lagtime extended from 306 s to 900 s by the compound with 0.15 mmol·L-1. The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that kojic acids was a competitive inhibitor for the oxidation of catechol, and the inhibition constant was 0.51 mmol·L-1.

Key words: Lymantria dispar, phenoloxidase, inhibitory mechanism, tetra-hexylresorcinol, kojic acids

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