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林业科学 ›› 2008, Vol. 44 ›› Issue (9): 26-30.doi: 10.11707/j.1001-7488.20080905

• 论文 • 上一篇    下一篇

外源GABA对NaCl胁迫下中间锦鸡儿幼苗乙烯生成的调控作用*

史胜青1,2 齐力旺1 肖文发2 李春秀2 林萍1 张守攻1

  

  1. (1. 中国林业科学研究院林业研究所 北京100091; 2. 中国林业科学研究院森林生态环境与保护研究所 北京100091)
  • 收稿日期:2007-10-12 修回日期:1900-01-01 出版日期:2008-09-25 发布日期:2008-09-25
  • 通讯作者: 张守攻

Role of γ Aminobutyric Acid (GABA) on Stimulating Ethylene Biosynthesisin Caragana intermedia Seedlings under NaCl Stress

Shi Shengqing1.2,Qi Liwang1,Xiao Wenfa2,Li Chunxiu2,Lin Ping1,Zhang Shougong1

  

  1. (1. Research Institute of Forestry, CAF Beijing 100091; 2. Research Institute of Forest Ecology, Enviroment and Protection, CAF Beijing 100091)
  • Received:2007-10-12 Revised:1900-01-01 Online:2008-09-25 Published:2008-09-25

摘要:

对NaCl胁迫条件下中间锦鸡儿幼苗施加外源GABA的研究表明:200 mmol\5L\+\{-1\} NaCl处理72 h时,幼苗体内乙烯含量增加,但是外源GABA能够进一步提高NaCl胁迫条件下乙烯含量; 在250 mmol·L-1 NaCl胁迫初(24 h)乙烯含量急剧增加,施加外源GABA反而抑制了乙烯的生成; 随着胁迫时间的延长,外源GABA对乙烯的生成表现为促进作用。转录水平研究进一步表明:NaCl胁迫条件下外源GABA提高了乙烯生成关键基因1-氨基环丙烷-1-羧酸(ACC)氧化酶基因[STBX]CaACO 1和CaACO 2[STBZ]在中间锦鸡儿根部的表达。

关键词: 中间锦鸡儿, NaCl胁迫, γ-氨基丁酸, 乙烯

Abstract:

Exogenous GABA was applied on Caragana intermedia seedlings under NaCl stress. The results showed that the plants treated with 200 mmol·L-1
significantly increase in ethylene production in 72 h, while exogenou
s GABA could further enhance the production of ethylene. At early stage treatments of 250 mmol·L-1NaCl and exogenous GABA, NaCltreated tissues showed obvious increase in ethylene production, whereas exogenous GABA inhibited the production of ethylene in the NaCltreated tissues. With the extension of treated time, exogenous GABA did promote the production of ethylene. At the transcriptional level, exogenous GABA induced more expression of aminocyclopropane1carboxylie acid (ACC) oxydase genes, [STBX] CaACO1 and CaACO 2 [STBZ] in C. intermedia that was under NaCl stress, compared with NaCl stress only. 

Key words: Caragana intermedia, NaCl stress, γ aminobutyric acid, ethylene