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林业科学 ›› 2008, Vol. 44 ›› Issue (9): 59-64.doi: 10.11707/j.1001-7488.200809011

• 论文 • 上一篇    下一篇

人心果品种资源亲缘关系的AFLP分析

文亚峰1 谢碧霞1 何 钢1 潘晓芳2   

  1. (1.中南林业科技大学资源与环境学院 长沙410004; 2.广西大学林学院 南宁530004)
  • 收稿日期:2007-11-20 修回日期:1900-01-01 出版日期:2008-09-25 发布日期:2008-09-25

AFLP Analysis of Genetic Relationship of Sapodilla Germplasm Resources

Wen Yafeng1,Xie Bixia1,He Gang1,Pan Xiaofang2   

  1. (1.College of Resources and Environment, Central South University of Forestry and Technology Changsha 410004; 2.College of Forestry, Guangxi University Nanning 530004)
  • Received:2007-11-20 Revised:1900-01-01 Online:2008-09-25 Published:2008-09-25

摘要:

利用荧光AFLP分子标记技术对27个人心果品种(类型)及4个蔓妹人心果品种资源的亲缘关系进行研究。9对引物(PstⅠ-MseⅠ)共扩增得到1 131条带(75 ~500 bp),其中多态性带1 096条(96.90%)。28个品种(类型)具特征性条带,特征带大小为350~500 bp品种间多态性比例在22.19%~45.89%之间,遗传相似性系数为0.40~0.87。UPGMA聚类结果将31个品种聚为蔓妹人心果类群(MAM)和2个人心果类群(SAP1和SAP2)。在遗传相似性系数0.52处, 4个蔓妹人心果品种首先被区分开,单独聚为一类(MAM)。人心果类群中, 11个国品种和10个国内地方类型在相似性系数0.66处聚为一大组(C、D组),相互之间表现出近的亲缘关系。大部分栽培品种聚于D2亚组,与地方类型有一定的遗传距离,说明栽培种的亲缘关系较为相近。人心果表型标记与分子标记结果间存在一定差异,二者的相关性不高

关键词: 人心果, 品种资源, 亲缘关系, 遗传多样性, AFLP

Abstract:

The genetic relationship of 27 sapodilla (Manilkara zapota) cultivars and 4 mamey sapoae (Pouteria sapota) cultivars were analyzed by using fluorescentlabeled AFLP markers. A total of 1 131 AFLP markers between 75 to 500 base pairs (bps) were generated with 9 primer combinations, of which 1 096 (96.9%) were polymorphic. Among 31 cultivars, 28 ones had unique bands ranging from 350 to 500 base pairs (bps).The percentage of polymorphic bands was from 22.19% to 45.89% in cultivar level. The genetic similarity coefficients (Nei and Li's) ranged from 0.40 to 0.87 With UPGMA clustering method, 31 cultivars were separated into three clusters. The 4 mamey sapoae cultivars were grouped in MAM. In sapodilla cluster (SAP1 and SAP2), 11 American cultivars and 10 Chinese types showed close genetic relationship and were grouped in 0.66 similarity coefficients (C and D clusters). Most culturing cultivars were grouped in D2 cluster and had a farther genetic distance from Chinese types or wild species, which indicated that culturingcultivars had close relationship. There was no significant correlation between UPGMA dendrograms of morphological and AFLP markers using the Mantel test.

Key words: Sapodilla (Manilkara zapota), cultivar resources, genetic relationship, genetic diversity, AFLP