欢迎访问林业科学,今天是

林业科学 ›› 2008, Vol. 44 ›› Issue (5): 79-83.doi: 10.11707/j.1001-7488.20080516

• 论文 • 上一篇    下一篇

细胞壁降解酶在落叶松-杨栅锈菌与寄主互作过程中的作用

田呈明1 赵鹏2 曹支敏2   

  1. (1.北京林业大学?省部共建森林培育与保护教育部重点实验室 北京100083;2. 西北农林科技大学林学院 杨凌712100)
  • 收稿日期:2006-11-15 修回日期:1900-01-01 出版日期:2008-05-25 发布日期:2015-04-22

Role of Cell Wall Degrading Enzymes in Interaction of Poplar and Melampsora laricipopulina

Tian Chengming1,Zhao Peng2,Cao Zhimin2   

  1. (1.The Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University Beijing 100083; 2.College of Forestry, Northwest A & F University Yangling 712100)
  • Received:2006-11-15 Revised:1900-01-01 Online:2008-05-25 Published:2015-04-22

摘要:

对落叶松-杨栅锈菌入侵不同抗性寄主过程中的细胞壁降解酶活性进行测定,明确聚甲基半乳糖醛酸酶(PMG)、果胶甲基反式消除酶(PMTE)、羧甲基纤维素酶(C-X)、β-葡萄糖苷酶(β-G)4种降解酶在该菌入侵中的作用。研究发现:抗病和感病组合中PMG均在接种后第2天和第5天出现2个活性高峰,在第5天时达到最大值。PMTE、C-X和β-G活性分别第3、2和3天达到活性高峰,其中PMG和PMTE的活性高,而C-X和β-G的活性则相对较低。在感病组合内PMG、PMTE、C-X、β-G活性较抗病组合大,表明这4种细胞壁降解酶对该菌入侵起一定的作用。

关键词: 落叶松-杨栅锈菌, 细胞壁降解酶, 杨树

Abstract:

The activities of cell walldegrading enzymes in poplar cultivars infected by Melampsora laricipopulinawere studied. The results showed that PMG, PMTE, C-X and β-glucosidase played roles during infection. After inoculation, the activity of PMG in both susceptible and resistant cultivars had two peak values respectively in 2 dpi and 5 dpi. The activities of PMTE and βglucosidase had their peak values in 3dpi, and C-X in 2 dpi. The activities of PMG and PMTE were higher while the activities of C-X and β-glucosidase were relatively lower. Activities of these cell walldegrading enzymes were significant higher in susceptible cultivars than those in resistant cultivars. The results suggested that these cell walldegrading enzymes played certain roles in infection of M. larici populina.

Key words: Melampsora laricipopulina, cell walldegrading enzymes, poplar