长春花花叶病病原物的分离和鉴定*

doi:10.11707/j.1001-7488.20080412

林业科学 ›› 2008, Vol. 44 ›› Issue (4): 60-68.doi: 10.11707/j.1001-7488.20080412

长春花花叶病病原物的分离和鉴定*

曹言勇¹ 施艳¹ 范在丰¹ 宋丽敏² 田国忠²

（1. 中国农业大学植物病理系农业部分子病理学重点开放实验室北京100094；2. 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京100091）

收稿日期:2007-02-09 修回日期:1900-01-01 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 田国忠

Isolation and Identification of the Pathogen That Causes Mosaic on Periwinkle

Cao Yanyong¹,Shi Yan¹,Fan Zaifeng¹,Song Limin²,Tian Guozhong²

(1. Key Lab. for Molecular Plant Pathology of the Ministry of Agriculture of PRC Department of Plant Pathology, China Agricultural University Beijing 100094; 2. Research Institute of Forest Ecology, Environment and Protection, CAF Key Lab. of Forest Protection， State Forestry Administration Beijing 100091)

Received:2007-02-09 Revised:1900-01-01 Online:2008-04-25 Published:2008-04-25

摘要/Abstract

摘要：

长春花病原物采自海南省琼海市博鳌镇和广东省广州市星海公园，症状表现为系统花叶与畸形，对其进行染病外植体组织培养、鉴别寄主反应、传播方式鉴定、病毒粒体形态观察和血清学反应及病叶双链RNA（dsRNA）分析。引起长春花花叶病的病原物鉴定为黄瓜花叶病毒（cucumber mosaic virus，CMV），克隆该病毒分离物外壳蛋白基因核苷酸序列，并对该核苷酸序列进行生物信息学分析，结果显示侵染我国长春花引起长春花花叶和畸形的CMV分离物应初步划归在CMV IB亚组。

关键词: 长春花, 花叶病, 黄瓜花叶病毒

Abstract:

The systemic mosaic and malformation symptoms were observed on the leaves of periwinkle (Catharanthus roseus) from Bo’ao, Qionghai, Hainan Province and Xinghai Park, Guangzhou, Guangdong Province. With the infected tissue culture, host range determination, serological assay and viral particle morphological observation, dsRNA analysis, the pathogen was identified to be cucumber mosaic virus (CMV). Cloning of the coat protein gene and analysis of the nucleotide sequences further demonstrated that this CMV isolate was tentatively assigned to the IB subgroup of CMV.

Key words: Catharanthus roseus, mosaic disease, cucumber mosaic virus（CMV）

曹言勇施艳范在丰宋丽敏田国忠. 长春花花叶病病原物的分离和鉴定*[J]. 林业科学, 2008, 44(4): 60-68.

Cao Yanyong;Shi Yan;Fan Zaifeng;Song Limin;Tian Guozhong. Isolation and Identification of the Pathogen That Causes Mosaic on Periwinkle[J]. Scientia Silvae Sinicae, 2008, 44(4): 60-68.

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长春花花叶病病原物的分离和鉴定*

Isolation and Identification of the Pathogen That Causes Mosaic on Periwinkle

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