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林业科学 ›› 2007, Vol. 43 ›› Issue (09): 128-132.doi: 10.11707/j.1001-7488.20070923

• 研究简报 • 上一篇    下一篇

Apl 基因转化地被菊品种‘玉人面’的研究*

吕晋慧1,2 吴月亮1 孙磊1 张启翔1   

  1. 1.北京林业大学园林学院 北京100083;2.山西农业大学林学院 太谷030801
  • 收稿日期:2006-09-19 修回日期:1900-01-01 出版日期:2007-09-25 发布日期:2007-09-25

Genetic Transformation of Chrysanthemum morifolium cv.‘Yu Ren Mian’with AP1 Gene Mediated by Agrobacterium tumefaciens

Lü Jinhui1,2,Wu Yueliang1,Sun Lei1,Zhang Qixiang1   

  1. 1.College of Landscape Architecture,Beijing Forestry Univenity Beijing 100083;2.College of Forest,Shanxi Agricultural University raigu 030801
  • Received:2006-09-19 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

关键词: 地被菊, Apl 基因, 根癌农杆菌, 遗传转化

Abstract:

The flower’s meristematic characteristic gene Apl was introduced into Chrysanthemum morifolium cv.‘Yu RenMian’mediated by Agrobacterium tumefaciens.The factors influencing genetic transformation protocol were studied.The resuhsshowed that the leaf explants precultured for 2~8 hours or not precuhured were best for transformation by A.Tumefaciens.The suitable concentration of bacterial and the time for infecting was OD600 0.5 for 10 minutes.Leaves were cocultivated with bacterial at 23~25℃ for 2 days.Then delayed selection for 3 days.Kanr plant were selected by increased selective press from 5 mg·L-1 G418 to 7.5 mg·L-1 G418 followed by 10 mg·-1 G418.The integration of Apl gene into C.Morifolium‘Yu Ren Mian’was confirmed by PCR and Southern blotting.Two of transgenic plants bloomed 15 days earlier than untransformed plants.

Key words: Chrysanthemum morifolium, Apl gene, Agrobacterium tumefaciens, genetic transformation