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林业科学 ›› 2007, Vol. 43 ›› Issue (01): 68-71.doi: 10.11707/j.1001-7488.20070111

• 论文及研究报告 • 上一篇    下一篇

杨树与杨盘二孢菌激发子互作进程中活性氧的释放及膜脂过氧化

冯莹1,2 韩正敏1   

  1. 1.南京林业大学,南京210037;2.广东省森林病虫害防治与检疫总站广州510173
  • 收稿日期:2005-07-05 修回日期:1900-01-01 出版日期:2007-01-25 发布日期:2007-01-25

Active Oxygen Release and Membrane Lipid Peroxidation in the Interaction of Marssonina brunnea Elicitor and Poplar Suspension Cell

Feng Ying1,2,Han Zhengmin1   

  1. 1.Nanjing Forestry University Nanjing 210037; 2.General Station of Forest Pest Management and Quarantine of Guangdong Province Guangzhou 510173
  • Received:2005-07-05 Revised:1900-01-01 Online:2007-01-25 Published:2007-01-25

摘要:

用杨盘二孢菌的激发子粗提液,与高度抗黑斑病的I-72杨和高度感染黑斑病的加杨悬浮细胞互作,测定杨树悬浮细胞的生物化学变化。结果表明:I-72杨在活性氧的释放和ΔpH的变化两方面均明显高出加杨;膜脂过氧化过程,从量的方面看,二者几乎没有差异,只是I-72杨的膜脂过氧化高峰期要早于加杨1h。这些研究可以为杨树的抗病机理和杨树抗病基因的研究提供基础。

关键词: 杨树, 杨生褐盘二孢菌, 悬浮细胞, 激发子, 病害互作

Abstract:

The biochemical changes of suspension cell of the susceptible poplar and the resistant one through the interaction between suspension cell of poplar and the crude elicitor of Marssonina brunnea were determined. The results are as below: the production of active oxygen and the ΔpH of suspension cell of I-72 poplar were higher than Populus canadensis in whole process. There was no distinct difference on the quantity in the membrane lipid peroxidation (MDA) of suspension cell of P. canadensis and I-72, but the MDA rush hour in I-72 appeared one hour earlier than in the P. canadensis poplar. These could provide a foundation to understanding the resistant mechanism and the resistant gene of poplar.

Key words: poplar, Marssonina brunnea, suspension cell, elicitor, disease interaction