关键词: 马尾松, 种子园, 遗传多样性, 父本分析, 微卫星(SSR)

Abstract:

Eleven microsatellite(SSR)markers were used to evaluate the genetic diversity and paternal parents of a seed orchard for Pinus massoniana planted at Wuyi Forest Farm,Fujian Province.The seed orchard contains 286 open-pollinated progenies derived from 129 candidate paternal clones and three maternal trees.Both the parental and progeny populations displayed high genetic diversity(h),with the estimated h values of 0.543 8 and 0.455 6,respectively.Paternal clones contributed differently to the progeny;only 5.3%～15.8% clones contributed 27.08%～48.96% pollen.More strikingly,one paternal clone contributed 10% and 17% pollen,respectively,to two maternal trees.It was found that the mating compatibility of a clone was not related to its origin.There was no significant influence of the physical location of a clone on the amount of paternal constituent and genetic diversity.The rate of contamination by pollen outside the seed orchard was as high as 9.44%.No selfing progeny was observed,suggesting that selfing depression will not be an issue in the seed orchard of P.massoniana.

Key words: Pinus massoniana, seed orchard, genetic diversity, paternity analysis, microsatellites(SSR)