转betA基因小黑杨的耐盐性分析及优良转基因株系的选择

doi:10.11707/j.1001-7488.20060706

林业科学 ›› 2006, Vol. 42 ›› Issue (7): 33-36.doi: 10.11707/j.1001-7488.20060706

转betA基因小黑杨的耐盐性分析及优良转基因株系的选择

刘桂丰¹ 杨传平¹ 蔡智军² 程贵兰² 詹立平¹

1.东北林业大学林学院,哈尔滨150040；2.辽宁农业职业技术学院,熊岳115214

收稿日期:2005-01-07 修回日期:1900-01-01 出版日期:2006-07-25 发布日期:2006-07-25

Salt Tolerance of betA Transgenic Populus simonii×P. nigra and Selection for Superior Transgenic Plants

Liu Guifeng¹,Yang Chuanping¹,Cai Zhijun²,Cheng Guilan²,Zhan Liping¹

1. College of Forestry, Northeast Forestry University Harbin 150040; 2. Liaoning Agricultural Professional and Technical College Xiongyue 115214

Received:2005-01-07 Revised:1900-01-01 Online:2006-07-25 Published:2006-07-25

摘要/Abstract

摘要：

在前期获得13个小黑杨转胆碱脱氢酶基因(betA)株系的基础上,选择9个生长正常的转基因株系与非转基因对照,用0、100、140、170、200mmol·L^-15种浓度的NaCl处理盆栽苗木,分2、7、12、17、22d取处理材料叶片,测定转基因株系叶片中外源基因的最终合成产物———甜菜碱含量。盐处理60d后,测定苗木高度及转基因株系的盐害指数。结果表明,转基因株系与对照间的甜菜碱含量差异达到极显著水平,9个转基因株系的甜菜碱平均含量超过对照的27.1%;转基因株系的平均盐害指数较对照低15.8%;转基因株系的平均高度超过对照44.1%。综合转基因株系的生长速度,兼顾其耐盐性,初步选择转基因株系T4、T6、T5、T1作为生长速度快耐盐性高的优良株系。

关键词: 小黑杨, 转基因株系, 胆碱脱氢酶基因(betA), 甜菜碱, 耐盐性

Abstract:

betA is a gene encoding choline dehydrogenase. In the previous work, 13 betA transgenic Populus simonii×P.nigra plants were obtained. In this experiment, 9 transgenic plants were chosen for salt tolerance test. Transgenic and untransformed control plants were treated with 0, 100, 140, 170, and 200 mmol·L^-1 NaCl for different period of time. After these plants were treated for 2, 7, 12, 17 and 22 days, the betaine content of transgenic plants and untransformed control plants were determined, respectively. After the transgenic and untransformed control plants were treated with salt for 60 days, plant height and index of salt damage were determined. The results of ANOVA and multiple comparisons showed that the betaine content between transgenic and untransformed control plants was statistically different. The betaine content in transgenic plants was 27.1% higher than that in untransformed control plants. The index of salt damage in transgenic plants was 15.8% lower than that in untransformed control plants. And the plant height of transgenic plants was 44.1% higher than that of untransformed control plants. Based on the growth rate and the salt tolerance, T1, T4, T5 and T6 transgenic lines were identified as plants with high salt tolerance and fast growth.

Key words: Populus simonii×, P. nigra, transgenic plant, betA, betaine, salt tolerance

刘桂丰杨传平蔡智军程贵兰詹立平. 转betA基因小黑杨的耐盐性分析及优良转基因株系的选择[J]. 林业科学, 2006, 42(7): 33-36.

Liu Guifeng;Yang Chuanping;Cai Zhijun;Cheng Guilan;Zhan Liping. Salt Tolerance of betA Transgenic Populus simonii×P. nigra and Selection for Superior Transgenic Plants[J]. Scientia Silvae Sinicae, 2006, 42(7): 33-36.

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转betA基因小黑杨的耐盐性分析及优良转基因株系的选择

Salt Tolerance of betA Transgenic Populus simonii×P. nigra and Selection for Superior Transgenic Plants

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