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林业科学 ›› 2008, Vol. 44 ›› Issue (3): 76-80.doi: 10.11707/j.1001-7488.20080317

• 论文 • 上一篇    下一篇

农杆菌介导BADH基因转化美丽胡枝子的研究

胡冬南1,2 陈晓阳2 李伟2 骈瑞琦2 赵杨3   

  1. 1.江西农业大学园林与艺术学院,南昌330045;2.北京林业大学林木花卉遗传育种教育部重点实验室,北京100083;3.贵州大学林学院,贵阳550025
  • 收稿日期:2007-01-25 修回日期:1900-01-01 出版日期:2008-03-25 发布日期:2008-03-25

Transformation of Lespedeza formosa with BADH Gene Mediated by Agrobacterium tumefaciens

Hu Dongnan1,2,Chen Xiaoyang2,Li Wei2,Pian Ruiqi2,Zhao Yang3   

  1. 1.College of Landscape Architecture and Art,Jiangxi Agricultural University Nanchang 330045; 2.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education Beijing Forestry University Beijing 100083; 3. Forestry College of Guizhou University Guiyang 550025
  • Received:2007-01-25 Revised:1900-01-01 Online:2008-03-25 Published:2008-03-25

摘要: 以子叶节为外植体,草丁膦为选择剂,对根癌农杆菌LBA4404介导的美丽胡枝子遗传转化进行研究,建立美丽胡枝子遗传转化体系,获得转BADH基因植株,最高转化率可达28%。抗性植株经分子检测表明,目的基因已整合进美丽胡枝子基因组中。NaCl胁迫下,转基因植株能积累更多的甜菜碱,证明BADH基因在转化植株中得到正常表达。

关键词: 美丽胡枝子, BADH基因, 基因转化, 甜菜碱

Abstract: The genetic transformation of Lespedeza formosa mediated by Agrobacterium tumefaciens was studied by using the cotyledonary nodes as explants and the Glufosinate(Glu) as a selector.The genetic transformation system of Lespedeza formosa was developed and the BADH transgenic plants were achieved. The highest transformation frequency was able to reach to 28%. The molecular analysis showed that the BADH gene was integrated into genome of L.formosa. The transgenic plants were able to accumulate more betaine than the untransformed ones under NaCl stress,which demonstrated the transgenic plants were able to express BADH gene normally.

Key words: Lespedeza formosa, BADH gene, gene transformation, betaine