欢迎访问林业科学,今天是

林业科学 ›› 2006, Vol. 42 ›› Issue (4): 122-125.doi: 10.11707/j.1001-7488.20060422

• 研究简报 • 上一篇    下一篇

麻竹种苗工厂化生产的技术体系

何凤发1 倪成1 江小华2 刘朝霞1   

  1. 1.西南农业大学农学与生命科学学院,重庆400716;2.重庆市绿川生物工程开发有限公司,重庆401520
  • 收稿日期:2003-06-16 修回日期:1900-01-01 出版日期:2006-04-25 发布日期:2006-04-25

The Technical System of the Mass Propagation of Dendrocalamus latiflorus

He Fengfa1,Ni Cheng1,Jiang Xiaohua2,Liu Zhaoxia1   

  1. 1.College of Agronomy and Life Sciences,Southwest Agricultural University Chongqing 400716; 2.Chongqing Luchuan Bioengineering Co. LTD Chongqing 401520
  • Received:2003-06-16 Revised:1900-01-01 Online:2006-04-25 Published:2006-04-25

关键词: 麻竹, 组织培养, 工厂化快速育苗

Abstract:

Newly grown branch buds on node of Dendrocalamus latiflorus were used as explants to induce buds in MS, 1/2 MS, and N6 media with different hormone combinations, and tube seedlings were obtained. In order to improve its reproductive coefficient, survival rate and rooting morphology, four aspects were tested: the number of the inoculated seedlings per cluster, multiplication culture, rooting culture and field transplanting. The results showed that the optimum medium for primary culture was MS+BA2.0 mg·L-1+IBA 0.5 mg·L-1; the reproductive coefficient reached 7.31 with sturdy seedlings during multiplication and rooting culture, if four seedlings were inoculated per cluster in MS+ BA2.0 mg·L-1 + IBA0.3 mg·L-1 medium; the optimal medium for rooting was 1/2MS+ IBA4.0 mg·L-1, with survival rate as high as 95%. The rooted seedlings were transplanted in the medium consisting of perlite+ earth+ cinders ash (1∶1∶1); After 3 to 7 days of hardening in the bottle and then growing for 10 to 15 days, 90% of the seedlings were survived. Through the above method, more than 2 million bamboo seedlings have been propagated so far.

Key words: Dendrocalamus latiflorus, tissue culture, rapid propagation in factory