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林业科学 ›› 2004, Vol. 40 ›› Issue (3): 71-74.doi: 10.11707/j.1001-7488.20040312

• 论文及研究报告 • 上一篇    下一篇

悬铃木体细胞胚胎发生及植株再生

范国强 黎明 贺窑青 蒋建平   

  1. 河南农业大学,郑州450002
  • 收稿日期:2002-09-26 修回日期:1900-01-01 出版日期:2004-05-25 发布日期:2004-05-25

Somatic Embryogenesis of Platanus orientalis and Its Plantlet Regeneration

Fan Guoqiang,Li Ming,He Yaoqing,Jiang Jianping   

  1. Henan Agricultural University Zhengzhou450002
  • Received:2002-09-26 Revised:1900-01-01 Online:2004-05-25 Published:2004-05-25

摘要:

以叶片为材料,研究了悬铃木的体细胞胚胎发生及其植株再生。结果表明,悬铃木幼苗叶片在WPM +0.1mg·L-1 IBA +5.0mg·L-1 BA培养基上的愈伤组织诱导率和胚性愈伤组织诱导率分别为100 %和89.1% ;25℃的温度有利于胚性愈伤组织在WPM +0.5mg·L-1 BA培养基上进行体细胞胚胎发生;黑暗低温处理(10℃) 3d不但可以促进体细胞胚胎的发生过程,而且可以提高其发生频率。

关键词: 悬铃木, 叶片, 愈伤组织, 体细胞胚胎发生, 植株再生

Abstract:

The somatic embryogenesis and plantlet regeneration from the leaf callus of Platanus orientalis were studied in this paper. The results indicated that the callus and embryogenic callus(EC) frequency on the WPM+0.1mg·L-1 IBA5.0mg·L-1 BA medium were 100% and 89.1% respectively. The procedure of somatic embryogenesis of EC on WPM0.5mg·L-1 BA medium at 25℃ might be promoted. Not only could the stages of somatic embryogenesis on all media in the experiment be promoted, but also the frequency of the somatic embryogenesis might be enlarged.

Key words: Platanus orientalis, Leaf, Callus, Somatic embryogenesis, Plantlet regeneration