关键词: 刺槐, 四倍体, 组织培养, 不定根, 解剖结构

Abstract:

The effect of NAA, IBA on rooting of shoots in test tubes of the tetraploid Robinia pseudoacacia was studied by the aid of the different test designs such as factorial experiment, regressive orthogonality test. The optimal medium was 1/2 MSIBA 0.4 mg·L^-1NAA 0.25 mg·L^-1sucrose 2%agar 0.6%. The adventitious root formation of shoots in test tube was also observed by means of the anatomical method. Latent root primordium was not found in shoots in test tubes. The induced root primordium which originated from the division and differentiation of pith ray cell developed into the adventitious roots.

Key words: Robinia pseudoacacia, Tetraploid, Tissue culture, Adventitious roots, Anatomical structure