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林业科学 ›› 2003, Vol. 39 ›› Issue (5): 29-36.doi: 10.11707/j.1001-7488.20030505

• 论文及研究报告 • 上一篇    下一篇

蒙古栎天然群体遗传多样性的AFLP分析

李文英 顾万春 周世良   

  1. 中国林业科学研究院科技信息研究所,北京100091;中国林业科学研究院林业研究所,北京100091;中国科学院植物研究所系统与进化植物学开放研究实验室,北京100093
  • 收稿日期:2002-12-06 修回日期:1900-01-01 出版日期:2003-09-25 发布日期:2003-09-25

AFLP ANALYSIS ON GENETIC DIVERSITY OF QUERCUS MONGOLICA POPULATIONS

Li Wenying,Gu Wanchun,Zhou Shiliang   

  1. Institute of Scientific and Technological Information, CAF Beijing100091;Research Institute of Forestry, CAF Beijing100091;Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences Beijing100093
  • Received:2002-12-06 Revised:1900-01-01 Online:2003-09-25 Published:2003-09-25

摘要:

以黑龙江省小兴安岭嘉荫、内蒙古大青沟和河北省雾灵山的3个蒙古栎群体及北京东灵山辽东栎群体为供试材料,用筛选出的4对荧光引物,对4个群体共计96个个体进行AFLP分析,每对AFLP引物扩增出63~113条,共得到346条多态带;群体特异带及群体间共有带的差异与分布揭示了群体间的遗传差异及相似性;蒙古栎遗传多样性主要存在于群体内,群体间的遗传分化系数Gst为0.077。蒙古栎在种级水平的遗传多样性参数略高于群体水平,多态带百分率P分别为96.8%、67.2 % ,有效等位基因数Ae分别为1.220、1.208,Nei基因多样性指数H分别为0.145、0.134,Shannon多样性指数I分别为0.246、0.208。东灵山辽东栎群体的P为67.6% ,Shannon多样性指数I为0.220 ,Nei基因多样性指数H为0.134。UPGMA聚类分析结果表明,蒙古栎自然群体间的遗传距离有随地理距离跨度增加而递增的趋势。蒙古栎遗传多样性偏低可能与其在历史上长期用作经济树种、人为干预和环境破坏较为严重、现存林分基本上为次生林等因素有关

关键词: 蒙古栎, 辽东栎, 群体, AFLP标记, 遗传多样性

Abstract:

Three Quercus mongolica populations located at Jiayin, Xiaoxing′anling Mountain in Heilongjiang Province, Daqinggou in Inner Mongolia Autonomous Region, and Wulingshan in Hebei Province, and one Q. liaotungensis (a variety of Q. mongolica) population at Donglingshan in Beijing, totally 96 individuals were selected and analyzed by amplifications using 4 pairs of AFLP primers screened, each primer pair produced 63~113 bands and 346 polymorphic bands were obtained.As for Q.mongolica at species level, the effective number of alleles per locus (Ae) was 1 220, the percentage of polymorphic band (P) was 96.8%, and the Nei gene diversity index (H) was 0 145, Shannon's information index (I) was 0 246. At population level, the estimates were Ae=1.208,P=67.2%, and H=0.134,I=0 208 . In contrast, the estimates for Q.liaotungensis were almost the same (Ae=1.5,P =67.6%, H=0.134, I=0.220 ). As for Q.mongolica, genetic differentiation coefficient among populations (Gst) was 0.077. According to the UPGMA cluster analysis, the genetic distance among Q.mongolica populations became larger with the increase of geographical distance. The low level of genetic diversity of Q.mongolica might relate to the long term utilization as economic tree species in history, comparatively serious disturbance and environmental damage by human beings, and secondary forests of the existed stands.

Key words: Quercus mongolica, Quercus liaotungensis, Population, AFLP marker, Genetic diversity