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林业科学 ›› 2003, Vol. 39 ›› Issue (3): 63-69.doi: 10.11707/j.1001-7488.20030310

• 论文及研究报告 • 上一篇    下一篇

水曲柳未成熟胚的离体培养研究

张惠君 罗凤霞   

  1. 沈阳农业大学,沈阳110161
  • 收稿日期:2001-07-31 修回日期:1900-01-01 出版日期:2003-05-25 发布日期:2003-05-25

STUDY ON IN VITRO CULTURE OF IMMATURE EMBRYO OF FRAXINUS MANDSHURICA

Zhang Huijun,Luo Fengxia   

  1. Shenyang Agricultural University Shenyang 110161
  • Received:2001-07-31 Revised:1900-01-01 Online:2003-05-25 Published:2003-05-25

摘要:

以沈阳农业大学植物园内30a生水曲柳(Fraxinus mandshurica)为试材。分别于2000年7月和8月取水曲柳幼果,用0.1%的HgCl2 进行表面消毒,在无菌条件下取出未成熟胚进行诱导培养。在胚成苗后进行继代培养和生根培养。培养结果表明:在水曲柳胚的发育初期,进行胚培养不易成功;但当幼胚分化成子叶和胚根时,进行胚培养,胚的成活率和成苗率均较高。不加任何激素的WPM、MS、DKW和SH培养基都可以作为水曲柳未成熟胚培养的培养基。水曲柳组培苗继代培养的外植体分化率低,最高只能达到2.125苗·月-1 (培养基为WPM +BA 1mg·L-1 + NAA 0.1mg·L-1)。水曲柳组培苗的生根较容易。用不含任何激素的WPM和QL基本培养基作为其生根培养基效果较好,生根率分别为60%和66%。

关键词: 水曲柳, 未成熟胚, 离体培养

Abstract:

In this study, experimental materials were taken from thirty-year-old trees of Fraxinus mandshurica Rupr. growing on arboretum of Shenyang Agriculture University. In July and August of 2000 respectively, immature pterodia of F. mandshurica were taken, then surface chemical treatment was made to them with 0.1% mercury bichloride, finally, under aseptic condition, immature embryos were taken out from pterodium and cultivated on initial media. After emergence of seedlings, shoot multiplication culture and rooting culture were made. The result of immature embryo culture showed that it was difficult to obtain sprouts from proembryos culture, however, survival rate and plant percent of immature embryo culture both were high, when embryo root and cotyledon had developed, and immature embryos cultivated on agar-solidified WPM media containing no plant growth regulators were highest, up to 100% and 88.5% respectively. And seedling cultivated on WPM media grew prolifically and average height of seedling was higher compared with that on other media. The shoot multiplication rate of embryo culture of F. mandshurica was low, only 2.125 seedlings per month, when explants were cultivated on WPM+ BA 1 mg·L-1 +NAA 0.1 mg·L-1. Rooting of F. mandshurica was easy. Rooting percentage of explants cultivated on QL and WPM media was 66% and 60% respectively.

Key words: Fraxinus mandshurica, Immature embryos, In vitro culture