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林业科学 ›› 2002, Vol. 38 ›› Issue (3): 42-48.doi: 10.11707/j.1001-7488.20020308

• 论文及研究报告 • 上一篇    下一篇

用水稻微卫星引物进行竹子分子系统学研究初探

李淑娴 尹佟明 邹惠渝 丁雨龙 黄敏仁   

  1. 南京林业大学,南京210037
  • 收稿日期:2000-09-11 修回日期:1900-01-01 出版日期:2002-05-25 发布日期:2002-05-25

PRELIMINARY STUDY ON MOLECULAR SYSTEMATICS OF BAMBOO BY SSR PRIMERS DERIVED FROM RICE

Li Shuxian,Yin Tongming,Zou Huiyu,Ding Yulong,Huang Minren   

  1. Nanjing Forestry University Nanjing210037
  • Received:2000-09-11 Revised:1900-01-01 Online:2002-05-25 Published:2002-05-25

摘要:

竹子和水稻在进化上有较近的亲缘关系,本文利用水稻的微卫星引物对竹子的分子系统学进行了初步研究。与传统的分类结果不同,研究发现巴山木竹属是与青篱竹属相关属分化较大的1个竹种,同时也证实了巴山木竹属作为1个单独的属是成立的。研究也明确了茶秆竹的分类学位置。本研究从1个不同的层面对青篱竹属相关属及属下一些竹种的关系提供了1个新的研究结果,为利用现代分子生物学技术对广义青篱竹属的系统学研究提供了1个借鉴。同时本研究对开展竹子的分子生物学研究提供了1个崭新的思路和方法。

关键词: 水稻, 微卫星, 青篱竹属, 分子系统学

Abstract:

The taxonomic disorder in genus of Arundinaria Michaux is the disputing focus in the taxonomic study of leptomorph bamboos.For a long time,there is no suitable strategy to study the genus of Arundinaria and the related genera.In this paper we presented a method of using the SSR primers of rice to settle the problem according to the near phylogenic relationship between bamboo and rice.Differing to the traditional taxonomy,it showed that there was a large differentiation beween genus of Bashania Keng f.et Yi and related genera of Arundinaria Michaux,and proved that Bashania Keng f.was regarded as a distinct and isolated genus.The taxonomic problem of Arundinaria amabilis (McClure) Keng f. was also discussed in this paper.It provided a new result for the relationship of the genus of bamboo related to Arundinaria Michaux from different aspects comparing to the traditional study.It also offered a reference for the phylogenic study of Arundinaria Michaux in a broad sense by modern molecular biotechnique.This study put forth new thought and strategy to carry out molecular biology research in bamboo.

Key words: Rice, SSR, Arundinaria, Molecular systematics