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林业科学 ›› 2002, Vol. 38 ›› Issue (2): 15-20.doi: 10.11707/j.1001-7488.20020204

• 论文及研究报告 • 上一篇    下一篇

长白落叶松几种酶活力及在种源早期选择中的应用

冯玉龙 姜淑梅 王文章 敖红   

  1. 河北大学,保定071002;中国科学院西双版纳热带植物园昆明分部昆明650223;河北大学,保定071002;东北林业大学,哈尔滨150040
  • 收稿日期:2000-11-09 修回日期:1900-01-01 出版日期:2002-03-25 发布日期:2002-03-25

ACTIVITY OF VARIOUS KINDS OF ENZYME IN LARIX OLGENSIS AND ITS APPLICATION IN EARLY PROVENANCE SELECTON

Feng Yulong,Jiang Shumei,Wang Wenzhang,Ao Hong   

  1. Hebei University Baoding071002;Northeast Forestry University Harbin150040
  • Received:2000-11-09 Revised:1900-01-01 Online:2002-03-25 Published:2002-03-25

摘要:

不同季节测定了8个种源长白落叶松4种酶活力,分析了它们与生长的关系。结果表明,生长快的种源过氧化物酶、多酚氧化酶活力弱,抗坏血酸氧化酶、谷氨酸脱氢酶活力强;生长慢的种源则相反,4种酶活力与长白落叶松的生长速率密切相关。不同季节各种酶活力的聚类分析结果相似,且与生长性状的聚类分析结果相一致。回归分析表明,过氧化物酶活力与长白落叶松的生长呈显著负相关,抗坏血酸氧化酶、谷氨酸脱氢酶活力与长白落叶松的生长呈显著正相关。多酚氧化酶活力与长白落叶松生长的负相关性较低。初步研究表明,过氧化物酶、多酚氧化酶、抗坏血酸氧化酶和谷氨酸脱氢酶可成为长白落叶松种源早期选择的生理生化指标。

关键词: 长白落叶松, 种源早期选择, 过氧化物酶, 多酚氧化酶, 抗坏血酸氧化酶, 谷氨酸脱氢酶

Abstract:

Activities of 4 kinds of enzyme in Larix olgensis were measured in different season, and their relationship with growth were analyzed. The results showed that the activity of poroxidase and polyphenol oxidase were weak, and those of ascorbic acid oxidase and glutamate dehydrogenase were strong in the provenances growing fast; while the situations were opposite in the provenances growing slowly. The activities of 4 kinds of enzyme correlated with growth closely in L.olgensis. The results of system clustering with 3 kinds of enzyme activity in different season as variable were similar, and were indentical with that with growth properties as variable. Regressive analysis showed that the activities of poroxidase and polyphenol oxidase correlated with growth negatively in L.olgensis, while those of ascorbic acid oxidase and glutamate dehydrogenase positively. Preliminary results indicated that the 4 kinds of enzyme maybe become physiology and biochemistry indices of early provenance selection in L.olgensis.

Key words: Larix olgensis, Early provenance selection, Poroxidase, Polyphenol oxidase, Ascorbic acid oxidase, Glutamate dehydrogenase