烟管菌降解木质素的条件优化及其对竹材的降解特性

doi:10.11707/j.1001-7488.LYKX20250745

Abstract

Abstract:

Objective: This research aims to optimise the cultivation conditions for lignin degradation by Bjerkandera adusta, and explore its degradation effects on bamboo, so as to provide a scientific basis for overcoming the recalcitrance of bamboo waste and enhancing its valorisation. Method: The lignin-degrading capacity of B. adusta was assessed by measuring the activities of ligninolytic enzymes. Single-factor experiments, Plackett-Burman design for the screening of significant variables, and Box-Behnken response surface method were employed to optimise the culture conditions for lignin degradation. In addition, a liquid-state fermentation system was established to evaluate the degradation effects of B. adusta on bamboo. Result: B. adusta mainly secreted laccase and highly active manganese peroxidase (MnP). The activities of laccase and MnP reached their maxima after 6 days of cultivation, attaining 69.13 and 329.02 U·mL^?1, respectively. Single-factor experiments and Plackett-Burman analysis identified the C/N ratio, initial pH value, and Mn²⁺ concentration as the key factors affecting lignin degradation by B. adusta. Response surface analysis revealed that the optimal cultivation conditions were a C/N ratio of 7.80, an initial pH value of 6.10, and an Mn²⁺ concentration of 20.10 μmol·L^?1. Under these conditions, the lignin degradation rate reached 45.52% after 9 days of cultivation, representing an increase of 36.61% compared with that obtained prior to optimisation. Furthermore, bamboo treated with B. adusta exhibited pronounced changes in chemical structure and surface morphology, with increased surface roughness and loosening of the surface structure, accompanied by the formation of numerous cavities. Conclusion: B. adusta is capable of secreting laccase and highly active MnP, which together confer strong lignin-degrading capacity under optimal cultivation conditions. Under the conditions of C/N 7.80, initial pH 6.10, and Mn²⁺ concentration of 20.10 μmol·L^?1, the fungus exhibits a significant degradation effect on bamboo and shows considerable potential application in biological pretreatment and valorisation of bamboo waste.

Key words: fungi, Bjerkandera adusta, lignin degradation, enzyme activity, optimization of degradation conditions, bamboo

CLC Number:

S718.81

Zhijie Zong,Zhiqiang Li,Jiayue Zhao. Optimization of Conditions for Lignin Degradation by Bjerkandera adusta and the Degradation Characteristics on Bamboo[J]. Scientia Silvae Sinicae, 2026, 62(9): 198-208.

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序号 No.	因素水平 Factor levels					木质素降解率 Lignin degradation (%)
序号 No.	X₁	X₂	X₃	X₄	X₅	木质素降解率 Lignin degradation (%)
1	?1	1	?1	1	1	34.36
2	?1	?1	?1	?1	?1	32.59
3	?1	1	1	1	1	32.40
4	1	?1	?1	?1	1	38.72
5	1	1	?1	1	?1	38.71
6	?1	?1	?1	1	?1	33.64
7	1	1	1	?1	?1	36.67
8	1	1	?1	?1	1	41.76
9	?1	1	1	?1	?1	33.40
10	1	?1	1	1	1	35.80
11	1	?1	1	1	?1	35.58
12	?1	?1	1	?1	1	30.72
P	0.000 1	0.023 1	0.004 1	0.357 7	0.385 0	—
R²	0.948 5	—	—	—	—	—

序号 No.	因素水平 Factor levels			木质素降解率 Lignin degradation (%)	方差来源 Source	平方和 Sum of squares	df	均方差 Mean square	F	P
序号 No.	A	B	C	木质素降解率 Lignin degradation (%)	方差来源 Source	平方和 Sum of squares	df	均方差 Mean square	F	P
1	?1	?1	0	40.49	A	2.06	1	2.06	10.29	0.014 9
2	1	?1	0	40.22	B	1.16	1	1.26	5.77	0.047 4
3	?1	1	0	39.65	C	3.38	1	3.38	16.87	0.004 5
4	1	1	0	42.44	AB	2.34	1	2.34	11.69	0.011 2
5	?1	0	?1	39.78	AC	1.42	1	1.42	7.07	0.032 5
6	1	0	?1	41.74	BC	0.504 1	1	0.504 1	2.52	0.156 7
7	?1	0	1	42.03	A²	40.57	1	40.57	202.50	<0.000 1
8	1	0	1	41.61	B²	18.46	1	18.46	92.16	<0.000 1
9	0	?1	?1	40.76	C²	9.52	1	9.52	47.54	0.000 2
10	0	1	?1	42.30	模型 Model	86.55	9	9.62	48.00	<0.000 1
11	0	?1	1	43.01	误差 Residual	1.40	7	0.200 3	—	—
12	0	1	1	43.13	失拟项 Lack of fit	0.245 1	3	0.081 7	0.282 3	0.836 5
13	0	0	0	45.92	纯误差 Pure error	1.16	4	0.289 3	—	—
14	0	0	0	46.36	总和 Total	87.95	16	—	—	—
15	0	0	0	45.50	R²	0.984 1	—	—	—	—
16	0	0	0	45.23	R²_adj	0.963 6	—	—	—	—
17	0	0	0	46.48	R²_pred	0.934 9	—	—	—	—

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Optimization of Conditions for Lignin Degradation by Bjerkandera adusta and the Degradation Characteristics on Bamboo

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