Abstract: Volatile and semi-volatile components of Paeonia veitchii roots were extracted respectively from steam and water residue by using water steam distillation, their antibacterial activity were evaluated against 6 bacteria (Escherichia coli, Staphylococcus aureus, Salmonella sp., Bacillus megaterium, Bacillus brevis, Pseudomonas aeruginosa), and the chemical compositions were analyzed by GC/MS. The results indicated that the volatile components contained 2-hydroxy-benzaldehyde (20.24%), benzoic-acid (21.64%), n-hexadecanoic acid (6.84%), oleic acid (8.76%), phenol (1.53%), methyl-salicylate (1.22%), furfural (2.49%), 2,2-dimethy1-3-octene (2.22%), et al., accounting for 97.18% of the volatile chemical components. The semi-volatile components contained 3,4,5-trihydroxy-benzoic acid (29.22%), 1,2,3-benzenetriol (14.00%), benzoic-acid (21.11%), 4-hydroxy-3-methoxy-benzoic acid (3.18%), 4-hydroxy-benzeneethanol (1.74%), 2-hydroxy-benzaldehyde (1.94%), furfural (2.89%), 1-(4-hydroxy-3-methoxy-pheny1)-ethanone (5.91%), 5-hydroxymethyl-2-furancarboxaldehyde (3.09%), salicy-alcohol (2.04%), (Z,Z)-9,12-octadecadienoic acid (3.82%), accounting for 95.19% of the semi-volatile chemical components. The volatile and semi-volatile components of Radix Paeoniae Rubra exhibited good activity against Staphylococcus aureus and Salmonella sp., and the semi-volatile components exhibited significant activity against Salmonella sp. in the terms of hypha growth rate.