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Scientia Silvae Sinicae ›› 2024, Vol. 60 ›› Issue (1): 93-102.doi: 10.11707/j.1001-7488.LYKX20230202

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Establishment of Symbiotic System of Piriformospora indica and Rhododendron and Its Effect on Improving Drought Resistance

Yue Fan,Peirun Luo,Wei Wang,Qian Xie,Qingxi Chen*()   

  1. College of Horticulture, Fujian Agriculture and Forestry University Fuzhou 350002
  • Received:2023-05-14 Online:2024-01-25 Published:2024-01-29
  • Contact: Qingxi Chen E-mail:.cqx0246@163.com

Abstract:

Objective: This study aims to establish a symbiotic system between Piriformospora indica and Rhododendron, in order to provide new ideas for improving the drought resistance of existing landscaping Rhododendrons. Method: A Rhododendron pulchrum cultivar, ‘Purple Butterfly’, commonly used in Chinese gardens was continuously irrigated with P. indica liquid for 6 times in a total of 30 days. A symbiotic system of P. indica and Rhododendron was established. The morphological and structural changes of Rhododendron plants colonized with P. indica were investigated, and the effects of P. indica on the drought resistance of Rhododendron and some related physiological and biochemical indexes were evaluated. Result: P. indica gradually colonized the Rhododendron root system after being irrigated with P. indica liquid for 4 times (on the 20th d). The colonization rate reached 91.67% after being irrigated with P. indica liquid for 5 times (on the 25th d) and 100% after being irrigated with P. indica liquid for 6 times (on the 30th d). After 30 days of colonization, the fresh weight, dry weight and leaf area increased, the thickness of the upper and lower epidermis of the leaves decreased, the ratio of palisade tissue to mesophyll increased, leaf architecture became more compact and root cell area increased. With extension of drought stress time, the growth status of Rhododendrons colonized with P. indica was improved. After 20 days of drought stress, the survival rate was high. There were no significant differences in relative electrical conductivity, proline content, malondialdehyde content, and superoxide dismutase activity of leaves with the extension of drought time. The half lethal time was significantly prolonged, and the degree of stress was light. The drought resistance was significantly enhanced. Conclusion: R. pulchrum can establish a mutually beneficial symbiotic relationship with P. indica, thereby improving its resistance to drought stress. It is believed that P. indica can be used as a microbial agent to improve the drought resistance of Rhododendron.

Key words: Rhododendron, Piriformospora indica, mycorrhizal-like fungus, symbiosis, drought resistance

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