Scientia Silvae Sinicae ›› 2024, Vol. 60 ›› Issue (1): 93-102.doi: 10.11707/j.1001-7488.LYKX20230202
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Yue Fan,Peirun Luo,Wei Wang,Qian Xie,Qingxi Chen*()
Received:
2023-05-14
Online:
2024-01-25
Published:
2024-01-29
Contact:
Qingxi Chen
E-mail:.cqx0246@163.com
CLC Number:
Yue Fan,Peirun Luo,Wei Wang,Qian Xie,Qingxi Chen. Establishment of Symbiotic System of Piriformospora indica and Rhododendron and Its Effect on Improving Drought Resistance[J]. Scientia Silvae Sinicae, 2024, 60(1): 93-102.
Table 1
Leaf morphological grade standard of Rhododendron under drought stress"
等级 Grade | 叶片生长情况 Leaf growth |
0 | 生长状态良好,无萎蔫现象出现 The growth state is good, no wilting phenomenon appears. |
1 | 幼叶反卷,10%以下叶片出现萎蔫、下垂、皱缩现象 The young leaves rolled back, leaves below 10% appear wilting, drooping and shrinking. |
2 | 幼叶反卷,10%~29%以下叶片出现萎蔫、下垂、皱缩现象 The young leaves rolled back, leaves below 10%-29% appear wilting, drooping and shrinking. |
3 | 幼叶反卷,30%~59%以下叶片出现萎蔫、下垂、皱缩现象 The young leaves rolled back, leaves below 30%-59% appear wilting, drooping and shrinking. |
4 | 幼叶反卷,60%以上叶片出现萎蔫、下垂、皱缩现象 The young leaves rolled back, more than 60% leaves appear wilting, drooping and shrinking. |
Fig.1
The colonization of P. indica A1 is the uncolonized root after Trypan blue staining; A2 is the colonized root after Trypan blue staining; B1 is the uncolonized root under scanning electron microscope; B2 is the colonized root under scanning electron microscope; C shows the molecular detection results of Pi group; D shows the colonization rate of P. indica; The different lowercase letters indicate a significant difference at 0.05 level. CK:对照control;Pi:印度梨形孢菌液处理P. indica liquid treatment."
Fig.2
Changes of leaf morphological structure after P. indica colonization A1 and A2 refer to the leaves treated with CK for 30 and 60 days, respectively; B1 and B2 refer to the leaves treated with P. indica liquid for 30 and 60 days; C1 and C2 refer to the cross-sectional anatomical structure of leaves treated with CK for 30 and 60 days; D1 and D2 refer to the cross-sectional anatomical structure of leaves treated with P. indica liquid for 30 and 60 days; E–N represents the LFW (leaf fresh weight), LDW (leaf dry weight), LA (leaf area), LI (leaf index), LT (leaf thickness), UET (upper epidermis thickness), LET (lower epidermis thickness), PT (palisade parenchyma thickness), ST (spongy parenchyma thickness), and P/S (palisade to spongy ratio of leaves treated with CK and P. indica liquid for 30 and 60 days, respectively."
Fig.3
Effect of colonization P. indica on root ultrastructure A is the root system of CK group ( left ) and Pi group ( right ) after 60 days of treatment; B is the thickness of the cell wall; C is the cross-sectional area of the cell; D1 and D2 are root cells of the CK group treated for 60 days, with magnifications of 2 500 and 7 000 times, respectively; E1 and E2 are root cells of the Pi group treated for 60 days, with magnifications of 2 500 and 7 000 times, respectively. CW: 细胞壁Cell wall; M: 线粒体Mitochondria; G: 高尔基体Golgi."
Fig.4
Effects of colonizing P. indica on plant drought resistance A: The grading of wilting damage of leaves in CK and Pi groups under different stress time was studied; B: The relative conductivity of CK and Pi group leaves under different stress time; C: The death rate of plants after 10 days of rewatering."
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