松材线虫对长白落叶松的致病性

doi:10.11707/j.1001-7488.20201111

Abstract

Abstract:

Objective: This study aims to provide theoretical basis on pathogenicity mechanism of Larix olgensis with pine wilt disease,and give some advice on pine wilt disease control in northern China. To this end,we investigated the pathogenicity and migration of pinewood nematode (PWN)Bursaphelenchus xylophilus in L. olgensis and the activity changes of superoxide (SOD) in L.olgensis after being inoculated with PWNs. Method: Two-year-old L. olgensis seedlings were inoculated sepa rately with three different isolates of PWN(QH-1,NM-1,and CM-1),the number of inoculation was 2 000 nematodes per seedling. After then,the pathogenicity,the external symptom and the nematode migration were observed daily. The stems segments with the same length above and below the inoculation spot(1.5 cm) were cut down in 1,2,4,7,10,15,and 25days after the inoculation,the cross-section was observed for detecting the disease occurrence and the migration of PWNs. Leaves were collected every day till 7days after inoculation,to analysis the change of SOD activity in L. olgensis. In 35 days after the inoculation,the morbidity was recorded and PWNs were extracted separately from wilt seedlings. The pathogenicity and migration of QH-1,NM-1,and CM-1 PWN in L. olgensis seedlings and the change of SOD activity in L. olgensis were analyzed. Result: L. olgensis seedlings were all wilt after inoculation with QH-1,NM-1 and CM-1 PWNs,but QH-1 PWNs caused seedlings to wilt 5days earlier than NM-1 and CM-1 PWNs. In 35 days after the inoculation,the morbidity rates of seedlings inoculated with QH-1,NM-1,and CM-1 PWN were 100%,70% and 60%,respectively,and the disease-infected indices were 100,43.3 and 40,respectively. The number of PWNs of QH-1 treatment was significantly greater than that of NM-1 and CM-1 treatments(P < 0.01). The migration ability of PWNs after inoculation showed the difference among QH-1,NM-1,and CM-1,and the migration ability of QH-1 was higher than that of NM-1,and CM-1. The change of SOD activity in L. olgensis showed the difference over QH-1,NM-1,and CM-1 PWN treatments,and the increase degree and decrease degree of QH-1 treatment were greater than those of NM-1 and CM-1 PWN treatments. The observation of cross-section showed that browning of pith and cavitation of xylem occurred at the early stage,and the QH-1 PWN treatment caused more serious damage than NM-1 and CM-1 PWN treatments. Conclusion: According to the external symptoms and the morbidities of seedlings,the migration of PWNs,the change of SOD activity,and the investigation of the cross-section,the three isolate PWNs all can cause wilt to L. olgensis seedlings,and the QH-1 PWN is more virulent than NM-1 and CM-1 PWN. Some of the inoculated seedlings had no obvious disease,but a small number of nematodes could be isolated,which indicates that the wilt of L. olgensis is related to the migration ability and fertility of the PWN inside L.olgensis.

Key words: Bursaphelenchus xylophilus, Larix olgensis, pathogenicity, inoculation, morbidity

CLC Number:

S763.11

Yefan Cao,Laifa Wang,Xizhuo Wang,Jiehong Fan. Pathogenicity of Bursaphelenchus xylophilus to Larix olgensis Seedlings[J]. Scientia Silvae Sinicae, 2020, 56(11): 108-115.

Figures/Tables 5

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References 0

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Pathogenicity of Bursaphelenchus xylophilus to Larix olgensis Seedlings

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线虫编号 Inoculum	接种株数 Number	首次发病时间 Time/d	发病率 Morbidityrate(%)	感病指数 Disease-infected index	再分离线虫量 Recovery of nematodes
QH-1	10	10	100	100	23 457±2 862a
NM-1	10	15	70	43.3	14 090±2 190b
CM-1	10	15	60	40	15 857±2 079b
CK	10	-	0	0	0

接种时间 Time/d	线虫编号 Inoculum	接种点方向 Direction	不同距离茎段线虫分布数量 The distribution and number of nematode in stems with different distance/cm
接种时间 Time/d	线虫编号 Inoculum	接种点方向 Direction	0.5~1.5	1.5~2.5	2.5~3.5	3.5~4.5	4.5~5.5	5.5~6.5	6.5~7.5
1	QH-1	+	5	2	0	0	0	0	0
	CM-1	-	32	34	24	10	5	0	0
	NM-1	+	0	0	0	0	0	0	0
		-	2	1	0	0	0	0	0
		+	0	0	0	0	0	0	0
		-	1	1	0	0	0	0	0
2	QH-1	+	2	1	0	0	0	0	0
	CM-1	-	20	15	25	10	5	1	0
	NM-1	+	0	0	0	0	0	0	0
		-	0	2	0	0	0	0	0
		+	0	1	0	0	0	0	0
		-	1	2	1	0	0	0	0
4	QH-1	+	4	8	6	4	1	0	0
	CM-1	-	5	16	10	20	8	19	10
	NM-1	+	1	1	0	0	0	0	0
		-	2	1	1	0	0	0	0
		+	2	1	0	0	0	0	0
		-	4	1	1	0	0	0	0
7	QH-1	+ -	30	24	26	15	13	5	2
	QH-1	+ -	42	36	32	34	28	30	22
	CM-1	+ -	10	5	3	0	0	0	0
	CM-1	+ -	12	8	5	2	0	0	0
	NM-1	+ -	10	5	3	0	0	0	0
	NM-1	+ -	12	8	5	2	0	0	0
10	QH-1	+	64	51	53	42	30	24	18
	CM-1	-	151	126	122	104	88	71	73
	NM-1	+	23	12	8	5	0	0	0
		-	32	21	15	10	3	0	0
		+	13	20	12	8	5	0	0
		-	24	26	18	12	9	5	0
15	QH-1	+	163	122	113	110	89	62	53
	CM-1	-	425	410	322	233	242	184	206
	NM-1	+	52	46	33	21	15	10	2
		-	86	63	55	50	35	24	13
		+	62	53	41	32	21	15	5
		-	93	76	62	53	43	30	27
20	QH-1	+	26	32	13	5	0	0	0
	CM-1	-	37	42	23	35	24	19	20
	NM-1	+	122	93	82	52	30	24	18
		-	151	126	122	104	88	71	73
		+	162	133	94	62	51	25	13
		-	213	176	132	93	73	61	57
25	QH-1	+	0	2	1	1	0	0	0
	CM-1	-	7	2	2	1	0	0	0
	NM-1	+	182	231	161	94	72	14	8
		-	291	282	213	195	103	82	64
		+	261	273	196	135	124	65	33
		-	192	281	237	173	126	91	65