3种绿僵菌对筛胸梳爪叩甲幼虫的毒力及保护酶活性的影响

doi:10.11707/j.1001-7488.20180612

Abstract

Abstract: [Objective] The bamboo wireworm (Melanotus cribricollis larvae) is one of the most rampant forest pests causing serious damage to bamboo shoots in southern China. It is hard to monitor and control this pest till now. Metarhizium spp. can be used for biocontrol of the wireworm. The purpose of this study is to screen high pathogenicity Metarhizium strain and explore the immune response of protective enzyme system in the wireworm after the fungal infection.[Method] The pathogenicity tests by applying three Metarhizium strains against the pest larvae with different spore concentrations were conducted. The dynamic activity changes of three protective enzymes in the host larvae, i.e. superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), were also determined during different infect periods.[Result] Results showed that all the three isolates possessed lethal property were able to infest M. cirbricollis larvae, among which M. pingshaense WP08 strain was the best, M. anisopliae WTKH strain was the second and M. acridum strain the last. The pathogenicity was enhanced with the increased spore concentration. With 10⁸spore·g^-1 DW soil, M. pingshaense WP08 strain exhibited the highest toxicity and all of the tested larvae died in 16 d, but M. pingshaense WP08 strain with 10⁷spore·g^-1 DW soil had no significant pathogenicity. There was difference in the dynamic change trends of three protective enzyme activities. There was no significant difference in the SOD activity. However, the CAT activity firstly down-regulated, then up-regulated and declined again subsequently, and the POD activity showed similar fluctuation trend to the CAT activity, but there was no significant difference at the late stage (5-7 d).[Conclusion] The present study indicated that M. pingshaense WP08 strain was the best entomopathogenic fungus to infect M. cribricollis larvae compared with other two strains. Therefore, the strain could be first selected as a biocontrol agent for control of the wireworm in bamboo forests. The fungal infection influenced activities of the three protective enzymes, especially to CAT activity.

Key words: larvae of Melanotus cribricollis, Metarhizium spp., pathogenicity, protective enzymes

CLC Number:

S763.306

Ye Bihuan, Zhang Yabo, Shu Jinping, Wu Hong, Wang Haojie. Effects of Three Different Metarhizium Strains on Virulence and Protective Enzymes Activities of Melanotus cribricollis larvae[J]. Scientia Silvae Sinicae, 2018, 54(6): 100-108.

References

曹传旺,高彩球. 2009. 昆虫生化与分子生物学实验技术. 哈尔滨:东北林业大学出版社.
(Cao C W, Gao C Q. 2009. Insect biochemistry and molecular biology experimental technique. Harbin:Northeast Forestry University Press.[in Chinese])
陈晨,蒋德春,杨洪,等. 2016. 南方水稻黑条矮缩病对褐飞虱和白背飞虱体内三种保护酶活性的影响. 环境昆虫学报,38(1):113-118.
(Chen C,Jiang D C,Yang H,et al. 2016. Effects of southern rice black streaked dwarf virus on defense enzymes in brown planthopper and white-black planthopper.Journal of Environmental Entomology,38(1):113-118.[in Chinese])
邓顺,舒金平,王浩杰. 2010. 筛胸梳爪叩甲幼虫寄主调查及其土壤空间分布. 昆虫知识,47(5):983-987.
(Deng S,Shu J P,Wang H J. 2010. Investigation of host rang of wireworms (Melanotus cribricolls) and their spatial distribution in soil. Chinese Bulletin of Entomology,47(5):983-987.[in Chinese])
付莉. 2014.黄褐天幕毛虫羧酸酯酶特性研究及杀虫剂对其酶活性的影响.哈尔滨:东北林业大学硕士学位论文.
(Fu L.2014. Study on characteristics of carboxylesterase and the effects of pesticides on several enzymatic activities in the Malacosoma neustria testacea.Harbin:MS thesis of Northeast Forestry University.[in Chinese])
韩永强,文礼章,侯茂林. 2016. 水稻施用硅肥对稻纵卷叶螟幼虫保护酶和解毒酶活性的影响. 应用昆虫学报,53(3):482-490.
(Han Y Q,Wen L Z,Hou M L,et al. 2016. Effects of providing additional silicon to host plants on the activity of protective and detoxifying enzymes in Cnaphalocrocis medinalis larvae. Chinese Journal of Applied Entomology,53(3):482-490.[in Chinese])
姬国红,钱秀娟,刘长仲,等. 2009. 夜蛾斯氏线虫对菜青虫几种保护酶活力的影响. 植物保护,35(4):66-69.
(Ji G H,Qian X J,Liu C Z,et al.2009. Changes of the activities of protective enzymes in Pieris rapae infected by Steinernema feltiae.Plant Protection,35(4):66-69.[in Chinese])
贾变桃, 焦鹏, 杨素梅. 2016. 虱螨脲亚致死浓度对小菜蛾保护酶系和解毒酶系活力的影响.植物保护学报,43(2):293-299.
(Jia B T,Jiao P,Yang S M.2016.Effects of sublethal concentrations of lufenuron on endogenous protective and detoxifying enzymes in the diamondback moth,Plutella xylostella (L.).Acta Phytophylacica Sinica,48(4):67-74.[in Chinese])
李会平,黄大庄,苏筱雨,等. 2007. 桑天牛幼虫感染白僵菌后体内主要保护酶活性的变化. 蚕业科学,33(4):634-636.
(Li H P,Huang D Z,Su X Y,et al. 2007. Change of activities of SOD,CAT and POD in Apriona germari larvae infected by Beauveria bassiana. Science of Sericulture,33(4):634-636.[in Chinese])
李品武,付雪莲,陈世春,等. 2016. 温度胁迫对茶淡黄刺蛾四种保护酶活力和总抗氧化力的影响. 应用昆虫学报,53(4):809-816.
(Li P W,Fu X L,Chen S C,et al. 2016. Effect of temperature stress on four protective enzymes and overall antioxidant capacity in Darna trima (Moore). Chinese Journal of Applied Entomology,53(4):809-816.[in Chinese])
李世广,窦婷婷,付小伶,等. 2016.菜青虫感染金龟子绿僵菌后体内几种保护酶活性的变化. 植物保护,42(3):133-136.
(Li S G,Dou T T,Fu X L,et al. 2016. Changes of protective enzyme activities in Pieris rapae infected by Metarhizium anisopliae. Plant Protection,42(3):133-136.[in Chinese])
李周直,沈惠娟,蒋巧根,等. 1994. 几种昆虫体内保护酶系统活力的研究. 昆虫学报,37(4):399-403.
(Li Z Z,Shen H J,Jiang Q G,et al. 1994. A study on the activities of endogenous enzymes of protective system in some insects. Acta Entomologica Sinica,37(4):399-403.[in Chinese])
刘丹, 严善春, 曹传旺,等. 2012. 多杀菌素对黄褐天幕毛虫解毒酶及保护酶的影响. 林业科学,48(4):67-74.
(Liu D,Yan S C,Cao C W,et al. 2012. Effect of spinosad on the detoxifying and protective enzymes of Malacosoma neustria testacea. Scientia Silvae Sinicae,48(4):67-74.[in Chinese])
刘玉坤, 王渭霞, 傅强,等. 2011.寄主植物对3种稻飞虱解毒酶和保护酶活性的影响. 中国水稻科学,25(6):659-666.
(Liu Y K,Wang W X,Fu Q,et al. 2011. Effects of host plants on activities of detoxification and protective enzymes in three rice Planthoppers.Chinese Journal of Rice Science,25(6):659-666.[in Chinese])
舒金平,滕莹,陈文强,等. 2012. 筛胸梳爪叩甲的防治技术研究. 林业科学研究,25(5):620-625.
(Shu J P,Teng Y,Chen W Q,et al. 2012. Control techniques of Melanotus cribricollis (Coleoptera:Elateridae). Forest Reaserch,25(5):620-625.[in Chinese])
王龙江,吕利华,谢梅琼,等. 2010. 红火蚁感染白僵菌后体内保护酶和酯酶活性的变化. 华中农业大学学报,29(3):282-286.
(Wang L J,Lü L H,Xie M Q,et al. 2010. Changes of activities of protective enzyme and detoxification enzymein Solenopsis invicta Buren infected by Beauveria bassiana. Journal of Huazhong Agricultural University,29(3):282-286.[in Chinese])
王梦龙,朱未,张继红,等. 2014. 氧化损伤是热胁迫下小金蝠蛾幼虫不能存活的重要原因. 昆虫学报,57(7):769-776.
(Wang M L,Zhu W,Zhang J H,et al. 2014. Oxidative damage is one of the most important causes of death of Thitarodes xiaojinensis (Lepidoptera:Hepialidae) larvae under heat stress. Acta Entomologica Sinica,57(7):769-776.[in Chinese])
王鹏,张亚波,舒金平,等. 2010. 金龟子绿僵菌小孢变种对筛胸梳爪叩甲幼虫致病力的生物测定. 中国生物防治,26(3):274-279.
(Wang P,Zhang Y B,Shu J P,et al. 2010. Virulence of Metarhizium anisopliae var. anisopliae to the larvae of Melanotus cribricollis (Coleoptera:Elateridae). Chinese Journal of Biological Control,26(3):274-279.[in Chinese])
王学奎. 2006. 植物生理生化实验原理和技术.2版. 北京:高等教育出版社.
(Wang X K. 2006. Principles and techniques of plant physiological biochemical experiment.2^nd Edition. Beijing:Higher Education Press.[in Chinese])
王莹.2016.6种杀虫剂对榆紫叶甲体内保护酶和解毒酶活性的影响. 哈尔滨:东北林业大学硕士学位论文.
(Wang Y.2016.Effect of six pesticides on the activities of the protective enzymes and detoxifying enzymes in Ambrostoma quadriimpressum Motsch.Harbin:MS thesis of Northeast Forestry University.[in Chinese])
徐天森,王浩杰. 2004. 中国主要竹子害虫. 北京:中国林业出版社.
(Xu T S,Wang H J. 2004. Main pests of bamboo in China. Beijing:China Forestry Publishing House.[in Chinese])
鄢杰明, 廖月枝, 严善春,等. 2010. 甲氧虫酰肼对舞毒蛾解毒酶和保护酶活性的影响.东北林业大学学报,38(11):112-114.
(Yan J M,Liao Y Z,Yan S C,et al. 2010. Effects of methoxyfenozide on the activities of detoxifying enzymes and protective enzymes in Lymantira dispar (Lepidoptera:Lymantriidae).Journal of Northeast Forestry University,38(11):112-114.[in Chinese])
叶碧欢,张亚波,舒金平,等. 2016. 竹林金针虫对绿僵菌及其代谢产物的行为反应. 生态学杂志,35(4):997-1002.
(Ye B H,Zhang Y B,Shu J P,et al. 2016. Behavioral responses of bamboo wireworm,Melanotus cribricollis to Metarhizium pingshaense and its metabolites. Chinese Journal of Ecology,35(4):997-1002.[in Chinese])
张傲. 2009. 重金属对麦长管蚜生态遗传学参数、保护酶活性、蛋白质含量及遗传进化的影响.杨凌:西北农林科技大学硕士学位论文.
(Zhang A. 2009. Effects of heavy metals on ecogenetic parameters,enzyme activity,protein level and evolution of the aphid. Yangling:MS thesis of Northwest A&F University.[in Chinese])
张奎花,钱秀娟,姬国红,等.2012.异小杆线虫对菜青虫体内保护酶活力的影响. 甘肃农业大学学报,47(2):58-61.
(Zhang K H,Qian X J,Ji G H,et al. 2012. Changes of the activities of protective enzymes in Pieris rapae infected by Heterorhabditis megidis. Journal of Gansu Agricultural University,47(2):58-61.[in Chinese])
张亚波,吴盼盼,王鹏,等. 2012. 一株绿僵菌的鉴定及其生物学特性. 林业科学,48(12):134-140.
(Zhang Y B,Wu P P,Wang P,et al. 2012. Identification and biological characteristics of a Metarhizium pingshaense strain isolated from Melanotus cribricollis larvae. Scientia Silvae Sinicae,48(12):134-140.[in Chinese])
朱建兰,王国利,刘谨,等.2008. 四脊裸胞壳dh菌株侵染对钩麦蛾幼虫体内保护酶的影响. 草地学报,16(2),121-124.
(Zhu J L,Wang G L,Liu J,et al. 2008. The protective enzyme activities of the larva of Aproaerema anthylidella Hübner infected by Emericella quadrilineata dh strain. Acta Agrestia Sinica,16(2):121-124.[in Chinese])
Amiri B,Ibrahim L,Butt T M. 1999. Antifeedant properties of destruxins and their potential use with the entomogenous fungus Metarhizium anisopliae for improved control of crucifer pests. Biocontrol Science and Technology,9(4):487-498.
Asi M R,Bashir M H,Afzal M,et al. 2013. Potential of entomopathogenic fungi for biocontrol of Spodoptera litura Fabricius (Lepidoptera:Noctuidae). Journal of Animal & Plant Sciences,23(3):913-918.
Ansari M A,Butt T M. 2013. Influence of the application methods and doses on the susceptibility of black vine weevil larvae Otiorhynchus sulcatus to Metarhizium anisopliae in field-grown strawberries. BioControl,2(58):257-267.
Dimbi S,Maniania N K,Ekesi S. 2013. Horizontal transmission of Metarhizium anisopliae in fruit flies and effect of fungal infection on egg laying and fertility. Insects,4(2):206-216.
Ding S Y,Li H Y,Li X F,et al. 2001. Effects of two kinds of transgenic poplar on protective enzymes system in the midgut of larvae of American white moth. Journal of Forestry Research,12(2):119-122.
Fridovich I. 1977. Oxygen is toxic. BioScience,27(7):462-466.
Genestra M. 2007. Oxyl radicals,redox-sensitive signaling cascades and antioxidants. Cellular Signalling,19(9):1807-1819.
Ho Y S,Xiong Y,Ma W,et al. 2004. Mice lacking catalase develop normally but show differential sensitivity to oxidant tissue injury. Journal Biological Chemistry,279(31):32804-32812.
Ibrahim A A,Mohamed H F,El-Naggar S E M,et al. 2016. Isolation and selection of entomopathogenic fungi as biocontrol agent against the greater wax moth,Galleria mellonella L. (Lepidoptera:Pyralidae). Egyptian Journal of Pest Control,26(2):249-253.
Kabaluk J T,Vernon R S,Goettel M S. 2007. Mortality and infection of wireworm,Agriotes obscurus (Coleoptera:Elateridae),with inundative field applications of Metarhizium anisopliae. Phytoprotection,88(2):51-56.
Kabaluk T. 2014. Targeting the click beetle Agriotes obscurus,with entomopathogens as a concept for wireworm biocontrol. BioControl,59(5):607-616.
Kabel A. 2014. Free radicals and antioxidants:role of enzymes and nutrition. World Journal of Nutrition and Health,2(3):35-38.
Lozinskaya Y L,Slepneva I A. 2004. Changes of the antioxidant status and system of generation of free radicals in hemolymph of Galleria mellonella larvae at microsporidiosis. Journal of Evolutionary Biochemistry and Physiology,40(2):119-125.
Meissle M,Pilz C, Romeis J. 2009. Susceptibility of Diabrotica virgifera virgifera (Coleoptera:Chrysomelidae) to the entomopathogenic fungus Metarhizium anisopliae when feeding on bacillus thuringiensis cry3bb1-expressing maize. Applied & Environmental Microbiology,75(12):3937-3943.
Pelizza S A,Scorsetti A C,Fogel M N,et al. 2015. Compatibility between entomopathogenic fungi and biorational insecticides in toxicity against Ronderosia bergi,under laboratory conditions. BioControl,60(1):81-91.
Roberts D W,St Leger R J. 2004. Metarhizium spp.,cosmopolitan insect-pathogenic fungi:mycological aspects. Advances in Applied Microbiology,54(1):1-70.
Roddam L F,Rath A C. 1997. Isolation and characterization of Metarhizium anisopliae and Beauveria bassiana from subantarctic Macquarie Island. Journal of Invertebrate Pathology,69(3):285-288.
Trevisoli A T,Trevisoli A L,Teixeira D R,et al. 2015. Efficiency of entomopathogenic fungi to the control of Sitophilus oryzae L. (Coleoptera:Curculionidae) under laboratory conditions. Comunicata Scientiae,6(1):90-96.
Wright C,Brooks A,Wall R. 2004. Toxicity of the entomopathogenic fungus,Metarhizium anisopliae (Deuteromycotina:Hyphomycetes) to adult females of the blowfly Lucilia sericata (Diptera:Calliphoridae). Pest Management Science,60(7):639-644.
Wu G,Yi Y,Lü Y,et al. 2015. The lipopolysaccharide (LPS) of Photorhabdus luminescens TT01 can elicit dose-and time-dependent immune priming in Galleria mellonella larvae. Journal of Invertebrate Pathology,127:63-72.
Yaninek J S,Herren H R. 1988. Introduction and spread of the cassava green mite,Mononychellus tanajoa Bondar.(Acari:Tetranychidae),an exotic pest in Africa and the search for appropriate control methods:a review. Bulletin of Entomological Reaearch,78(1):1-13.
Zelko I N,Mariani T J,Folz R J. 2002. Superoxide dismutase multigene family:a comparison of the CuZn-SOD (SOD1),Mn-SOD (SOD2),and EC-SOD (SOD3) gene structures,evolution,and expression. Free Radical Biology & Medicine,33(3):337-349.

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Effects of Three Different Metarhizium Strains on Virulence and Protective Enzymes Activities of Melanotus cribricollis larvae

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