大巴山区核桃实生居群的坚果表型和遗传多样性

doi:10.11707/j.1001-7488.20160513

Abstract

Abstract:

[Objective] Daba mountains is one of the most abundant areas of biodiversity and walnut (Juglans regia) resources because of its unique geography and climate. In recent years, walnut resources lose dramatically in this area due to human intervention. In view of the serious situation, investigations were carried out on the nut phenotypic diversity and genetic diversity of walnut populations originated from seedlings in Daba mountains to know its nut phenotypic variation, germplasm characteristics and genetic structure. [Method] Variation of 12 nut phenotypic traits (30 representative nuts per sample) and 16 gene loci (1 seedling per sample and 16 SSR primers were used) of 386 samples from 12 seedling populations in Daba mountains were analyzed. [Result] 1) The variation coefficient of nut phenotypic traits of 12 seedling populations in Daba mountains was between 26.75%-34.36%, with an average of 30.60%. And the germplasm resource of small nut (less than 6 g) accounted for a certain proportion. The Shannon index of the populations was between 1.112-1.546, with an average of 1.324, and the expected heterozygosity ranged from 0.612 to 0.718, with an average of 0.688, suggesting abundant variations in nut phenotypic and genetic diversity in this area. 2) The phenotypic and genetic differentiation among populations in this area was small, with differentiation coefficients of 8.15% and 6.32% respectively. 3) Cluster analysis showed that the Manhattan distance of nut phenotypic traits and the genetic similarity coefficient were positively related to the geographic distance. [Conclusion] There were large nut phenotypic diversity and genetic diversity in walnut trees in Daba mountains because of long-term regenerations from seedlings and adaptation to diverse site conditions. The Daba mountains is an important area of genetic diversity of walnuts in China. This study would provide an important basis for the walnut breeding and conservation in the region.

Key words: Juglans regia, populations, phenotype traits, genetic traits, diversity

CLC Number:

S718.46

Xu Yongjie, Han Huabai, Wang Hua, Chen Lingna, Ma Qingguo, Pei Dong. Phenotypic and Genetic Diversities of Nuts of Walnut(Juglans regia) Populations Originated from Seedlings in Daba Mountains[J]. Scientia Silvae Sinicae, 2016, 52(5): 111-119.

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Phenotypic and Genetic Diversities of Nuts of Walnut(Juglans regia) Populations Originated from Seedlings in Daba Mountains

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