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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (4): 32-38.doi: 10.11707/j.1001-7488.20140405

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Analysis of Selfing Depression in Liriodendron with SSR Markers

Pan Wenting1,2, Yao Junxiu1, Li Huogen1   

  1. 1. Nanjing Forestry University Nanjing 210037;
    2. Subtropical Experimental Center, Chinese Academy of Forestry Fenyi 336600
  • Received:2013-05-31 Revised:2013-07-09 Online:2014-04-25 Published:2014-05-06
  • Contact: 李火根

Abstract:

The existing Liriodendron has a small population size, and hence easily occurs selfing depression. In this study, we analyzed selfing depression of Liriodendron by taking 5 selfing progenies as experimental populations, 4 intra-species progenies and 8 inter-species progenies as reference populations. The phenotypic traits such as survival rate and growth were used as evaluation index, combined with SSR molecular marker, to analyze the extent of selfing depression in Liriodendron. The aim of this study is to explore the underlying mechanism of selfing depression. The results showed that the selfing depression in Liriodendron chinense was obvious. By taking progenies of intra-species cross and inter-species cross as references, the degree of depression (δ value) of selfing progenies for DBH, height and survival rate were 0.46, 0.45; 0.32, 0.35; and 0.25, 0.30 respectively. SSR test results showed that the homozygous progeny rate derived from different mating types of selfing, intra-species, inter-species was from high to low trend. Moreover, we also detected 2 candidate SSR loci that might relate to recessive lethal gene in Liriodendron.

Key words: Liriodendron, selfing depression, SSR, recessive lethal gene

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