甜樱桃矮化砧‘吉塞拉6号’扦插过程中氧化酶活性和碳氮含量变化

Abstract

Abstract:

In this paper, we investigated the rooting characteristics of the softwood cutting of 'Gisela 6'(Prunus cerasus × P. canescens)and the changes in activity of peroxidase (POD), polyphenoloxidase (PPO), indoleacetic acid oxidase (IAAO), total soluble sugar content, total nitrogen content and C/N ratio during the rooting. The results showed that:The rooting percentage, the root number and the average root length increased significantly when treated by IBA. The treatment of 1 000 mg·L^-1 IBA had the best effect on the rooting. The rooting percentage, the root number and the average root length were 81.2%, 10.5 and 2.95 cm, respectively. Compared with the control, the rooting percentage, the root number and the average root length increased by 133.3%, 262.1% and 45.3%, respectively. The activity of PPO, POD and IAAO, total soluble sugar content and C/N ratio changed regularly during the rooting. The activity of PPO and POD increased during the adventitious root induction and initiation, reached to a peak level on day 18, and then decreased during the root elongation period. However, the activity of IAAO decreased during the induction and initiation phase and increased slowly in the root elongation phase. The total soluble sugar content and C/N ratio increased during the induction phase, and then decreased in the following days. The total nitrogen content presented a continued decrease in the whole rooting process. The activity of PPO and POD enhanced significantly and changed more sharply during the rooting period after treatment with IBA. The IBA treatment also enhanced the total soluble sugar content and C/N ratio, but the total nitrogen content did not changed significantly. These results indicated that the activity of PPO, POD and IAAO, the total soluble sugar content and C/N ratio were related to the development of adventitious root closely. IBA increased rooting activities of the Gisela 6 softwood mainly owing to the increase of the activity of PPO and POD, the total soluble sugar content and C/N ratio and the reduction of the IAAO activity.

Key words: ‘Gisela 6’(Prunus cerasus×, P. canescens), softwood cutting, IBA, adventitious root development, oxidases, nutrients

CLC Number:

S723.13

Wei Hairong, Chen Xin, Zong Xiaojuan, Wang Jiawei, Zhang Qingxia, Zhang Daohui, Liu Qingzhong. Fluctuations of Oxidase Activities and Carbon and Nitrogen Content during the Rooting Process of Sweet Cherry Dwarf Rootstock ‘Gisela 6’ Softwood Cuttings[J]. Scientia Silvae Sinicae, 2013, 49(9): 172-177.

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Fluctuations of Oxidase Activities and Carbon and Nitrogen Content during the Rooting Process of Sweet Cherry Dwarf Rootstock ‘Gisela 6’ Softwood Cuttings

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