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Scientia Silvae Sinicae ›› 2015, Vol. 51 ›› Issue (5): 87-94.doi: 10.11707/j.1001-7488.20150510

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Inhibitive Activity of Chemical Constituents from Leaves of Pleioblastus amarus against Bacteria and 3 Kinds of Tumor Cells

Wei Qi, Yao Xi, Sun Jia, Xun Hang, Yue Yongde, Tang Feng   

  1. Key Laboratory of Bamboo and Rattan Science and Technology International Center for Bamboo and Rattan Beijing 100102
  • Received:2013-12-31 Revised:2014-04-21 Online:2015-05-25 Published:2015-06-11

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Abstract:

【Objective】 The compounds from leaves of Pleioblastus amarus were extracted and isolated. The inhibitive activity of the compounds against bacteria and 3 kinds of tumor cells were tested in vitro. The extract was extracted from the bamboo leaves with 95% ethanol and separated with the column chromatograph. The antibacterial activities of the extract against Escherichia coli, Staphyloccocus aureus and Bacillus subtilis were tested by the inhibition zone method. The inhibitive activity against tumor cells of 5,7,3'-trihydroxy-6-C-β-D-digitoxopyranosyl-4'-O-β-D-glucopyranosyl flavonoside was evaluated by the MTT method and the ascites tumor model of mice H22 hepatoma cells. 【Result】 The results showed that the antibacterial activity of ethyl acetate extracts was stronger than that of petroleum ether extracts and n-butanol extracts, while the water extracts had no obvious antibacterial effect. The antibacterial activity of Fr.3 was strongest in 8 fractional parts of ethyl acetate extracts. At the concentration of 25 mg·mL-1, Fr.3 showed the strongest antibacterial activity against Bacillus subtilis, and the diameter of inhibition zone was 21.05 mm in 24 h. At the concentration of 25 mg·mL-1, the Fr. 4 of ethyl acetate extracts had the strongest antibacterial activities against Escherichia coli and Staphyloccocus aureus of β-coumaric acid in all compounds, and the diameters of inhibition zone were 20.23 mm and 18.35 mm respectively in 24 h. High concentration of 5,7,3'-trihydroxy-6-C-β-D-digitoxopyranosyl-4'-O-β-D-glucopyranosyl flavonoside showed antitumor effects on Hela cells, HepG2 cells and HT-29 cells in vitro, and the IC50was 592 mg·L-1, 2 058 mg·L-1and 1 712 mg·L-1, respectively. The doses of 5 mg·kg-1and 10 mg·kg-15,7,3'-trihydroxy-6-C-β-D-digitoxopyranosyl-4'-O-β-D-glucopyranosyl flavonoside had antitumor effects on the liver tumors of H22 mice in vivo, and the inhibition rates were 2.9% and 20.0%, respectively. 【Conclusion】 Chemical Constituents from leaves of P. amarus have antibacterial and antitumor activities, and they have potential in the development of plant original pesticide and medicine. This study provides references for promoting the basic research and comprehensive utilization of P. amarus leaves.

Key words: Pleioblastus amarus, chemical components, antibacterial activity, antitumor activity

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