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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (6): 45-51.doi: 10.11707/j.1001-7488.20130607

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Dynamic of Physiology and Biochemistry during Wild Rhododendron scabrifolium Cutting Propagation

Zhao Yunlong1, Chen Xun2, Li Chaochan3   

  1. 1. College of Forestry,Guizhou University Guiyang 550025;
    2. Guizhou Academy of Sciences Guiyang 550001;
    3. School of Life Sciences,Guizhou Normal University Guiyang 550001
  • Received:2012-08-08 Revised:2013-01-26 Online:2013-06-25 Published:2013-07-16

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Abstract:

To establish a commercial feasible cutting propagation system of wild Azalea (Rhododendron scabrifolium), physiological mechanisms during the rooting of R. scabrifolium cutting propagation were investigated. Semi-hard wood stems of R. scabrifolium were collected and treated by KNAA and KIBA. Contents of endogenous hormones IAA, GA3, ZR, and ABA were measured during the rooting period, and soluble sugar, total nitrogen content and the oxidase activity were also determined in stems during cuttings. The results indicated that KNAA 2 000 mg·L-1+KIBA 2 000 mg·L-1 had a significant effect on rooting and resulted in the highest rooting rate of 78.65%. During the rooting process, the higher concentration of endogenous IAA favored the formation of root primodia, GA3 content showed a positive correlation with the cuttings callus induction and adventitious root formation, and low levels of ABA should promote rooting of cuttings. Endogenous ZR was needed for root primodium differentiation and callus formation. IAA/ABA ratio was smaller in the process of rooting, which might be one of the reasons that R. scabrifolium cuttings usually are difficult to root. The increase in soluble sugar content and reduction of nitrogen concentrations were both conducive to the generation of cuttings adventitious roots. Oxidase activity changed regularly in the process of cuttings rooting. The activity of SOD, POD, and PPO increased in the callus induction period, and slightly decreased after callus formation. POD and PPO activity in the root, increased at adventitious root formation stage; POD and PPO activity increased in the adventitious root formation period, and CAT activity remained at a high level after the root formation.

Key words: Rhododendron scabrifolium, cutting, endogenous hormones, nutrients, oxidases

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