Abstract:

Abstract: The embryogenic callus of Tripterygium wilfordii was used to study the effect of its subculture duration on the plant regeneration, and the effect of basic medium with different auxin concentrations and their combination with cytokinin on the proliferation of its regenerated plantlets. The results showed that during the process of subculture of the embryogenic callus, the regeneration rate and the number of the regenerated plantlets increased in the first stage and then declined in the second stage. The embryogenic callus with its subculture age between 8 month and 1 year old was most regenerative, with 100% regeneration rate and more plantlet number per callus. The callus had lost its ability to differentiate completely after 20 months of subculture age. The differentiated plantlets from calli were vulnerable to mutate. The plantlets could be divided into 6 types, according to the shapes of leafs of the plantlets. When the plantlets were propagated with stem sections, 1/2MS supplemented with 2.0 mg L^-1 IBA should be optimal. And 1/2MS+2.0 mg L^-1 IBA+0.5 g L^-1 active carbon medium was favorable for the plantlets to root, with rooting rate 100%. When transplanted in the mixture of perlite and humus (1∶1) soil, its survival rate was as high as 96%.

Key words: Key words: Tripterygium wilfordii, Embryogenic callus, tissue culture, rapid-propagation