Abstract:

The cutting propagation technique and relevant oxidase changes of Corylus heterophylla were studied with a L16（4.2×2.9） orthogonal experiment design which consisted of three factors of varieties, auxins(IBA, αNAA) and their concentrations. The activities of peroxidase(POD), polyphenol oxidase(PPO) and indoleacetic acid oxidase(IAAO) during rooting were investigated in this paper. The results showed that the cutting rooting rate was mainly affected by auxin mass fraction，following by the variety and the auxin type among the tested factors. Percentage of rooting reached to maximum under 0.03% of IBA. Various varieties had significant effect on rooting index, with Barcelona the maximum. The adventitious rooting was obtained in three distinct phases i.e. callus formation, induction and expression. The peroxidase activity in IBAtreated cuttings increased in the callus formation phase and declined in the expression phase. Polyphenol oxidase activity in IBAtreated cuttings increased during callus formation and induction phase, however declined slowly during expression phase. Indoleacetic acid oxidase activity of IBAtreated cuttings increased during callus formation phase and decreased during induction and expression phases.

Key words: Corylus heterophylla, rooting of cuttings, oxidases