Abstract:

Cuttings propagation technology and related oxidase changes of Corylus avellana Barcelona were studied with media and auxin comparison experiments. The dynamic change of activities of peroxidase (POD), polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO) during rooting were also investigated. The results showed that only the mixed media can enhance the percentage of cuttings rooting in the media experiment. Rooting percentage of cutting with 200×10^-6 IBA treatment was 60%, rooting percentage of cutting with 200×10^-6 α-NAA or ABT₁ treatment was 15% and 20%, the cuttings of control did not root. The adventitious rooting displayed three distinct phases I.e. induction, expression and elongation. The peroxidase activity in IBA-treated cuttings increased during induction and expression phase declined in the elongation phase. There were two peaks of the POD activity respectively on the 20th and 60th during rooting, Polyphenol oxidase activity in IBA-treated cuttings increased during induction phase, and then declined slowly during expression and elongation phases. Indoleacetic acid oxidase activity of IBA-treated cuttings increased slowly during induction phase and then increased sharply during expression phases and decreased during elongation phases.

Key words: Corylus avellana, hardwood cutting, rooting, oxidases