松茸SCAR标记的建立

doi:10.11707/j.1001-7488.20071028

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (10): 150-153.doi: 10.11707/j.1001-7488.20071028

Establishment of SCAR Marker Linked to Tricholoma matsutake

Wu Songquan¹ Guan Qingjie² Quan Xueli¹ Fu Weijie¹ Wu Jiri¹

1. Agricultural College of Yanbian University Longjing 133400; 2.Northeast Forestry University Harbin 150040

Received:2006-06-04 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

Abstract

Abstract:

RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphic band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from the polymorphic band by cloning, sequencing and designing primers. The SCAR marker only presented in the individuals of T. matsutake, thus, it can quickly and accurately identify and T. bakamatsutake at molecular level.

Key words: Tricholoma matsutake, Tricholoma bakamatsutake, RAPD, SCAR

Wu Songquan Guan Qingjie Quan Xueli Fu Weijie Wu Jiri. Establishment of SCAR Marker Linked to Tricholoma matsutake[J]. Scientia Silvae Sinicae, 2007, 43(10): 150-153.

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Establishment of SCAR Marker Linked to Tricholoma matsutake

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