菌根真菌液泡的分离及特性研究*

Abstract

Abstract:

The vacuoles were for the first time isolated from the mycorrhizal fungi (Amanita muscaria and Cenococcum geophilum) by the polybase, DEAED-dextron, induced lysis of protoplasts. The protoplasts isolated from mycelium cultured in MMNC+P medium for 4-7 days showed considerable vacuolation.The yield of vacuole was in the range of 5-10% of protoplasts. The purity of vacuolar preparation was judged by measuring and comparing soluble cytoplasmic enzyme G6PDH, which indicated that it was not contaminated by the cytoplasm and other cell organelles. The measurement of activities of acid phosphatase and α-mannosidase in equal number of vacuoles and protoplasts revealed that almost all this two enzymes were located in the vacuole, so they might be established as the vacuolar marker enzymes for A. muscaria and C.geophilum.

Key words: Amanita muscaria, Cenococcum geophilum, Mycorrhizal fungi, Vacuolar isolation

Fanxiang Kong. ISOLATION AND PROPERTIES OF VACUOLES FROM MYCORRHIZAL FUNGI[J]. Scientia Silvae Sinicae, 1996, 32(4): 342-347.

Figures/Tables 2

References 16

1	Suvercha, Mukerji K G. Ectomycorrhiza. In: Arora D K, Rai B, Mukerji K G et al. ed. Handbook of applied my-cology (soil and plants). 1991: 187-211
2	Mikio N , Harry B . Hydrolases in vacuoles from castor bean endosperm. Plant Physiol, 1978, 62 (1): 44- 48 doi: 10.1104/pp.62.1.44
3	Valk H D , Plegt L M , Loon L C V . Isolation of vacuoles from developing oat leaves. Plant Science, 1987, 52 (1): 159- 167
4	Martinoia E , Heck U , Boller T et al. Some properties of vacuoles isolated from Neurospora crassa slime variant. Arch Microbiol, 1979, 120 (1): 31- 34 doi: 10.1007/BF00413268
5	Durr M , Boller T , Wiemken A . Polybase induced lysis of spheroplasts. Arch Microbiol, 1975, 105 (1): 319- 327 doi: 10.1007/BF00447152
6	Keller U , Madry N , Kleinkauf H. et al. lsolation and characterization of vacuoles from the ergot fungus Claviceps purpurea. Applied and Environ Microbiol, 1984, 47 (2): 710- 714
7	Wiemken A , Durr M . Characterization of amino acid polls in the vacuolar compartment of Saccharomyces cerevisiae. Arch Microbiol, 1974, 101 (1): 45- 57 doi: 10.1007/BF00455924
8	Indge K J . Metabolic lysis of yeast protoplasts. J Gen Microbiol, 1968, 51 (1): 433- 440
9	Guttenberger M , Hampp R . Ectomycorrhizins-symbiosis-specific or artifactual polypeptides from ectomycorrhizas. Planta, 1992, 188 (1): 129- 136 doi: 10.1007/BF01160722
10	Chen X Y , Hampp R . Sugar uptake by protoplasts of the ectomycorrhizal fungus Amanita muscaria. New Phytologist, 1994, 125 (2): 601- 608
11	Hampp R , Einigw , Keil R . Profile biochemischere Parameter in pflanzlichen Geweben und Zellen und Zellen mit unterschiedlich ausgeprag ten Schadsy mptomen Kerforschungszentrum Karlsruhe. KfK-PEF, 1987, 32: 1- 48
12	John B W H, Glucose-6-phosphatase dehydrogenase from Agaricus bisporus: purification and properties J G Microbiol. 1985, 131(1): 321-328
13	Boller T , Kender H Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol, 1979, 63 (3): 1123- 1132.
14	Jones J G K. Out law W. Enzymatic assay for sucrose. In: Kornberg H L ed, Technique in carbohydrate metabolism. Amsterdam Elasevier, 1981: 1-8
15	Guttenberger M , Hampp R . A dot-blot assay for quantitation of nanogram amounts of protein in the presence of carrier ampholytes and other possibly interfering substances. Anal Biochem, 1991, 196 (1): 99- 103 doi: 10.1016/0003-2697(91)90124-C
16	Harris S D , Cotter D A . Transport of yeast vacuolar trehalase to the vacuole. Can J Microbiol, 1988, 34 (2): 835- 838

[1]	Xin Cheng,Chunze Wu,Qingyu Wei,Wei Li,Xing Wei. Growth and Physiological Responses of Fraxinus mandshurica Seedlings Inoculated with Arbuscular Mycorrhizal Fungi to Drought Stress [J]. Scientia Silvae Sinicae, 2023, 59(2): 58-66.
[2]	Di Huang,Yuan Chen,Luolong Zhong,Jiajun Liang,Zhengmu Wang,Zujing Chen. Growth and Defense-related Enzymes of Eucalyptus in Responses to Funneliformis mosseae and Ralstonia solanacearum [J]. Scientia Silvae Sinicae, 2023, 59(11): 68-75.
[3]	Xiao Wang,Yinli Bi,Yi Wang,Ye Tian,Qiang Li,Xinpeng Du,Yun Guo. Effects of Planting Density of Hippophae rhamnoides and Inoculation of AMF on Understory Vegetation Growth and Soil Improvement [J]. Scientia Silvae Sinicae, 2023, 59(10): 138-149.
[4]	Longfei Hao,Tingyan Liu,Yongqin He,Shengxi Zhang,Yuan Zhao. Responses of Rhizosphere Soil Stoichiometry of Clematis fruticosa Inoculated with Arbuscular Mycorrhizal Fungi to Nitrogen Deposition [J]. Scientia Silvae Sinicae, 2022, 58(6): 151-160.
[5]	Lina Han,Xianan Xie,Hui Chen,Ming Tang. Molecular Characteristics and Function of the Metal Tolerant Protein, EgMTP6 in Eucalyptus grandis [J]. Scientia Silvae Sinicae, 2022, 58(5): 93-101.
[6]	Juan Song,Zhuhua Wu,Xingliang Weng,Xing Zhao,Xuexiang Yang,Ronglin Tang,Bing Cao,Yu Wu,Houyu Shen,Jiahong Ren,Fengmao Chen. Diversity of Arbuscular Mycorrhizal Fungi in Rhizosphere of Liquidambar formosana [J]. Scientia Silvae Sinicae, 2021, 57(9): 98-109.
[7]	Peishan Zhao,Mishan Guo,Guanglei Gao,Guodong Ding,Ying Zhang. Characteristics of Community Structure and Functional Group of Fungi in Roots of Pinus sylvestris var. mongolica in the Horqin Sandy Land [J]. Scientia Silvae Sinicae, 2020, 56(9): 87-96.
[8]	Wang Wei, Hu Kai, Dang Chengqiang, Tao Jianping. Interaction of Litter Decomposition and Fine-Root Growth [J]. Scientia Silvae Sinicae, 2016, 52(4): 100-109.
[9]	Li Qian, Wu Xiaoqin, Ye Jianren. Isolation and Identification of a Mycorrhiza Helper Bacteria(MHB)—Brevibacillus reuszeri on Pinus massoniana Roots [J]. Scientia Silvae Sinicae, 2015, 51(5): 159-164.
[10]	Wang Lin, Chen Zhan, Shang He. Effects of Ectomycorrhizal Fungi(Pisolithus tinctorius) of Masson Pine (Pinus massoniana)on Soil Microbial Metabolic Function under Simulated Acid Rain [J]. Scientia Silvae Sinicae, 2014, 50(7): 99-104.
[11]	Song Fuqiang, Kong Xiangshi, Li Jize, Chang Wei. Screening the Related Genes in the AM Fungi and Amorpha fruticosa Symbiosis with the Suppression Subtractive Hybridization Technique [J]. Scientia Silvae Sinicae, 2014, 50(11): 64-74.
[12]	Wang Mingxia;Yuan Ling;Zhou Zhifeng;Yang Hongjun;Huang Jianguo. Efflux of Oxalate and Uptake of Nitrogen, Phosphorus and Potassium by Ectomycorrhizal Fungal Isolates in Vitro in Response to Aluminum Stress [J]. Scientia Silvae Sinicae, 2012, 48(2): 82-88.
[13]	Li Dengwu;Xue Ling;Zhang Wanhong. Diversity and Distribution of Arbuscular Mycorrhizal(AM) Fungi in Loess Hilly Gully Region [J]. Scientia Silvae Sinicae, 2011, 47(7): 116-122.
[14]	Song Ge;Song Fuqiang. Response Characteristics of Physiological Defense Indexes of Host Plant at Early Stage of Arbuscular Mycorrhiza Development between Amorpha fruticosa and AM Fungi [J]. Scientia Silvae Sinicae, 2011, 47(10): 44-50.
[15]	Sun Yue;Zhuang Haifeng;Jia Shuxia;Gu Jiacun;Wang Zhengquan. Effects of Nitrogen Fertilization on VA Mycorrhizal Fungal Colonization and Root Morphology of the First Order Roots in Fraxinus mandshurica Plantation in Response to the colonization [J]. Scientia Silvae Sinicae, 2010, 46(9): 50-57.

ISOLATION AND PROPERTIES OF VACUOLES FROM MYCORRHIZAL FUNGI

RichHTML

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

Figures/Tables 2

References 16

Related Articles 15

Recommended Articles

Metrics

Comments