近成熟期定额灌水对香榧假种皮开裂的解剖学变化及基因表达的影响

doi:10.11707/j.1001-7488.LYKX20240088

Abstract

Abstract:

Objective: The mechanism of quota water addition on the the aril cracking of Torreya grandis cv.‘Merrilii’ in the near mature period was discussed, and the theoretical basis for promoting the cracking rate of aril and reducing the harvesting cost was provided. Method: During the near maturity stage, by setting the quota water addition (the soil moisture content is approximately 26.5% at a depth of 25 cm) and control treatment (without water addition), then measuring the aril cracking rate, firmness of T. grandis cv.‘Merrilii’ in different periods, combined with paraffin sections observe the anatomic structure and analyzing the ethylene production volume and the cell wall components, and based on the transcriptome and RT-qPCR analysis to further reveal the intrinsic mechanism of water addition promotes the aril cracking. Result: 1) Water addition treatment significantly increased the cracking rate of the aril in T. grandis cv.‘Merrilii’ to 72.55%, decreased the firmness by 40.40% ( P ≤ 0.05 ), and increased the ethylene release by 1.32 times. The microstructure showed that the resin canals in the parenchyma cell layer of the aril treated with irrigation were obviously broken and separated, and the thickness of the parenchyma tissue layer increased significantly by 20.12%. 2) Water addition treatment significantly increased the water-soluble pectin (WSP) in aril, while the contents of CDTA soluble pectin (CSP), cellulose and hemicellulose decreased significantly. The cracking rate of aril was significantly positively correlated with WSP and significantly negatively correlated with CSP. 3) The expression levels of cell wall metabolism-related genes TgEXPs, TgPMEs and ethylene synthesis-related genes TgACOs were significantly up-regulated after irrigation treatment. Among them, the expression levels of TgEXP1, TgEXP2, TgPME1, TgPME2 and TgACO1 were significantly negatively correlated with firmness and CSP, but were significantly positively correlated with cracking rate, ethylene release and WSP. Conclusion: In the near mature period, quota water addition significantly promoted the cracking of the aril of T. grandis cv.‘Merrilii’ and shortened the cracking period by increasing the ethylene release and changing the cell wall components and related gene expression, which provided a reliable theoretical basis for the cultivation measures to improve the cracking rate.

Key words: Torreya grandis cv.‘Merrilii’, soil water content, aril cracking, cell wall metabolism, gene expression

CLC Number:

S722

Cuiping Wu,Caoliang Jin,Jianping Ying,Jinwei Suo,Jiasheng Wu,Yuanyuan Hu. Effects of Quota Water Addition on Anatomical Changes and Gene Expression in Aril Cracking of Torreya grandis cv. ‘Merrilii’ During Near Maturity Stage[J]. Scientia Silvae Sinicae, 2025, 61(1): 115-125.

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基因 Gene	上游引物 Forward primers (5'-3')	下游引物 Reverse primer (5'-3')
Q-TgEXP1	GCGCGTAGCTGCTTCTATTA	AGCGTAAGAGGCCCATATCT
Q-TgEXP2	AAGAAGCTTCGTTGACCCG	CCGAGTTGAGCCATTACGG
Q-TgEXP3	AAAATGGGCATCCTACGCTC	TCCATTAACCATCCCAGGGTA
Q-TgEXP4	TGCAGTGTTGCTGTTTCTCA	CGCCATAAAATGTAGCGTGC
Q-TgEXP5	CGCCAAATATCGACCTGGAA	GCCAAACTAATGCAGCGTAG
Q-TgEXP6	TCTGTGGTTATCTCCTCGTCT	CATCGCTGCCACCATAGAA
Q-TgEXP7	AGCTGTACTCAAGACTGCTCT	ATTGCTAAGATACCCACCAGG
Q-TgEXP8	TGGCCTTTCTCTGTGGGTAA	ATCACCGCCCCCATAAAATG
Q-TgPME1	TAATCCGAGCTACTGAGGCT	ACTATCAATGCCGAAGCCAG
Q-TgPME2	TTCTTGGCTAGGTTCGATGC	TACGCCATTACGCTCCTCTA
Q-TgACO1	GGAACAGTGAAGCCCTGAAG	GGATGTTGCCCTCTCAGAAA
Q-TgACO2	TGCCTCCCTTTTCTGGTTTG	CATTTGCAATTTGGGCCATGA

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Effects of Quota Water Addition on Anatomical Changes and Gene Expression in Aril Cracking of Torreya grandis cv. ‘Merrilii’ During Near Maturity Stage

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