基于培养性质模糊解析和28S rDNA-PCR-RFLP解析的外担子菌属真菌的分类学研究*

Abstract

Abstract:

The fungus belonging to the genus Exobasidium (Basidiomycotina, Ustomycetes, Exobasidiales) is known as the pathogen of cell's hyperplastic and hypertrophic disease of several tree plants. In this study, thirty-five isolates (isolated from Japan) of twelve species and one variety of the genus were divided into eighteen groups based on the fuzzy analysis of cultural properties (shapes and characters of colonies; growth curve of the isolates under different temperatures, pHs, nitrogen sources and carbon sources) and the analysis of 28S rDNA-PCR-RFLP. Among the eighteen groups, nine groups were identical with the species identified by the traditional taxonomy. These species are E.vexans Mass、E.reticulatum Ito et Sawada、E.camelliae Shirai、E.cylindrosporium Ezuka、E.shiraianum P. Heen.、E.hachijoense Otani, Kakishima et Iijima、E.bisporum Sawada et Ezuka、E.pievidis-ovalifoliae Sawada and E.symploci-japonicae Kusano et Tokubuchi. Tested seven isolates of E.gracile (Shirai) Sydow were differentiated into four groups that showed the differentiation related to geographical environment and hosts in the species. Tested seven isolates of E.japonicum Shirai were differentiated into five groups that showed the differentiation related to hosts in the species. Tested isolates of E.yoshinagai P. Heen., E.otanianum Ezuka var. satsumense Zhang et Arai and the three isolates of tested seven isolates of E.japonicum were classified into one group.

Key words: Exobasidium, Cultural properties, RFLP, Fuzzy analysis, Taxonomy

Xingyao Zhang. A STUDY ON THE TAXONOMY OF EXOBASIDIUM SPP. ACCORDING TO THE FUZZY ANALYSIS OF CULTURAL PROPERTIES AND THE ANALYSIS OF 28S rDNA-PCR-RFLP[J]. Scientia Silvae Sinicae, 1998, 34(4): 59-71.

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References 16

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A STUDY ON THE TAXONOMY OF EXOBASIDIUM SPP. ACCORDING TO THE FUZZY ANALYSIS OF CULTURAL PROPERTIES AND THE ANALYSIS OF 28S rDNA-PCR-RFLP

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