云杉属花粉形态的电镜扫描研究

doi:10.11707/j.1001-7488.20140507

Abstract

Abstract:

Pollen grains from 20 species of the genus Picea were examined using the scanning electron microscope, including P. retroflexa, P. koraiensis, P. asperata, P. meyeri, P. crassifolia, P. wilsonii, P. likiangensis, P.likiangensis var. balfouriana, P. purpurea, P. jezoensis var. microsperma, P. neoveitchii, P. schrenkiana var. tianschanica, P. schrenkiana, P. smithiana and P. likiangensis var. linzhiensis in China; P. polita in Japan, P. abies and P. omorika in Europe; and P. glauca and P. mariana in North America. The characteristics of pollen morphology among the species were distinguished using data statistics and photographic analysis. Mean pollen size in the 20 species was from 84.33 to 118.79 μm. According to morphological photography, corpus surface texture of pollens had two types, with or without grains distributing; and two bladders distributed at zygomorphic underside of corpus was found to being distinct edge of cap at near surface in corpus. Its surface texture was smoother and there was no distinct variation among species. Germinal furrow formed clear striate between two bladders. Results showed that the pollen morphology had variability among species, and had conservative within species, which would be a more intuitive and effective palynological index for phylogeny study in Picea.

Key words: Picea, pollen morphology, taxonomy, electron microscope scanning

CLC Number:

S718.47

Jia Zirui, Wang Junhui, Zhang Shougong, Ma Jianwei, Yang Guijuan. Pollen Morphology of 20 Species in Picea by Scanning Electron Microscope[J]. Scientia Silvae Sinicae, 2014, 50(5): 49-61.

References

蔡萍,宛涛,张洪波,等. 2009. 沙地云杉与其近缘种花粉形态的比较研究. 内蒙古大学学报,40(6): 685-672.
(Cai P, Wan T, Zhang H B, et al. 2009. Pollen morphology comparison of Picea mongolica(H.Q.Wu.) W.D.Xu with its close relative species. Journal of Inner Mongolia University, 40(6): 685-672. [in Chinese] )
贾子瑞,张守攻,王军辉. 2011. 7种云杉属树种花粉形态特征及其系统学的意义. 电子显微学报,30(3): 257-264.
(Jia Z R, Zhang S G, Wang J H. 2011. Morphology character and systematic significance of pollen in 7 spruce species. Journal of Chinese Electron Microscopy Society, 30(3): 257-264. [in Chinese] )
贾子瑞. 2011. 云杉属系统进化学研究. 北京: 中国林业科学研究院博士学位论文.
(Jia Z R. 2011. Study on the phylogeny of Picea. Beijing: PhD thesis of Chinese Academy of Forestry. [in Chinese] )
张金谈. 1899. 中国松科花粉形态研究.植物研究,9(3):87-98.
(Zhang J T. 1899. Studies on the pollen morphology of pinaceae in China. Bulletin of Botanical Research, 9(3):87-98. [in Chinese] )
郑万钧. 1978. 中国植物志. 北京: 科学出版社.
(Zheng W J. 1978. Flora reipublicae popularis sinicae. Beijing: Science Press. [in Chinese] )
周正,陈喜军,薛茂贤. 1987. 世界主要用材树种概论.北京:中国林业出版社.
Bagnell Jr C R. 1975. Species distinction among pollen grains of Abies, Picea, and Pinus in the rocky mountain area (A scanning electron microscope study). Review of Palaeobotany and Palynology, 19(3): 203-220.
Bouillé M, Senneville S, Bousquet J. 2011. Discordant mtDNA and cpDNA phylogenies indicate geographic speciation and reticulation as driving factors for the diversification of the genus Picea. Tree Genetics & Genomes, 7:469-484.
Cai M, Zhu H, Wang H. 2008. Pollen morphology of the genus Lasianthus (Rubiaceae) and related taxa from Asia. Journal of Systematics and Evolution, 46 (1): 62-72.
Chung K S, Elisens W J, Skvarla J J, et al. 2010. Pollen morphology and its phylogenetic significance in tribe Sanguisorbeae (Rosaceae). Plant Systematics and Evolution, 285(3/4):139-148.
Fukuda T, Naiki A, Nagamasu H. 2008. Pollen morphology of the genus Skimmia (Rutaceae) and its taxonomic implications. Journal of Plant Research, 121(5):463-471.
Liu T S. 1982. A new proposal for the classification of the genus Picea. Acta Phyt Geobot, 33: 227-244.
Lu L, Wang H, Blackmore S, et al. 2007. Pollen morphology of the tribe Rhinantheae (Orobanchaceae) and its systematic significances. Plant Systematics and Evolution, 134(4):527-533.
Lü S Y, Li Y F, Chen Z K, et al. 2002. Pollen development in Picea asperata Mast. Flora-Morphology, Distribution, Functional Ecology of Plants, 198(2): 112-117.
Nakagawa T, Edouard J L, de Beaulieu J L.2000. A scanning electron microscopy (SEM) study of sediments from Lake Cristol, southern French Alps, with special reference to the identification of Pinus cembra and other Alpine Pinus species based on SEM pollen morphology. Review of Palaeobotany and Palynology, 108(1/2): 1-15.
Nakagawa T, Yasuda Y, Tabata H. 1995. Pollen morphology of Himalayan Pinus and Quercus and its importance in palynological studies in Himalayan area. Review of Palaeobotany and Palynology, 91(1-4): 317-329.
Ran J H, Wei X X, Wang X Q. 2006. Molecular phylogeny and biogeography of Picea (Pinaceae): implications for phylogeographical studies using cytoplasmic haplotypes. Molecular Phylogenetics and Evolution, 41(2):405- 419.
Schmidt P A. 1989. Beitrag zur systematik und evolution der gattung Picea A. Dietr. Flora (Jena), 182(5/6):435-461.
Tellería M C, Katinas L. 2009. New insights into the pollen morphology of the genus Mutisia (Asteraceae, Mutisieae). Plant Systematics and Evolution, 280:229-241.
Tjoelker M G, Boratynski A, Bugala W. 2007. Biology and ecology of Norway spruce. Dordrecht, The Netherland:Spring, 23.
Walker J W. 1974. Evolution of exine structure in the pollen of primitive angiosperms. American Journal Botany, 61:891-902.
Wodehouse R P. 1935. Pollen grains. New York:Mc Graw Hill Book Co. Inc, 323-340.

[1]	Guo Hongyan, Wang Sen, Yan Chao, Zhang Zhen, Huang Wen. Characteristics of Male Flower and Its Pollen of Schisandra sphenanthera [J]. Scientia Silvae Sinicae, 2016, 52(3): 112-120.
[2]	Guo Kai, Gu Jianfeng, Wang Jiangling, Hu Jiafu. Redescription of Devibursaphelenchus hunanensis (Yin et al., 1988) (Nematoda: Ektaphelenchinae) from Pinus massoniana in China with the Synonymy of D. eproctatus (Sriwati et al., 2008) [J]. Scientia Silvae Sinicae, 2014, 50(7): 82-89.
[3]	Jia Jiwen;Ma Wenjun;Wang Junhui;Zhang Jinfeng;Zhang Shougong;Zhang Jianguo;Zhao Kun. Pollen Morphology of Several Species in Catalpa and Its Taxonomic Significance [J]. Scientia Silvae Sinicae, 2012, 48(7): 182-185.
[4]	Wan Huimin;Li Yonghua;Yang Qiusheng. Classification of Wintersweet Cultivars by Pollen Morphology [J]. Scientia Silvae Sinicae, 2012, 48(1): 91-96.
[5]	Yang Qiusheng;Wan Huimin;Sun Junya;Gong Shuangjun. Comparison of Pollen Morphology of Tree Peony Cultivar Groups [J]. Scientia Silvae Sinicae, 2010, 46(6): 133-137.
[6]	Yao Yanxia;Yang Zhongqi;Yan Jiahe. A New Species of the Genus Systasis Walker， 1834 (Hymenoptera： Pteromalidae) Parasitizing an Important Invasive Alien Insect Pest Obolodiplosis robiniae (Diptera： Cecidomyiidae) from China [J]. Scientia Silvae Sinicae, 2009, 12(8): 88-90.
[7]	Zheng Lin;Chen Hong;Zhang Lei;Zang Dekui. Pollen Morphology and Cultivar Classification of the Genus Chaenomeles [J]. Scientia Silvae Sinicae, 2008, 44(5): 53-57.
[8]	Zhou Lanying;Wang Yongqing;Zhang Li. Pollen Morphology and Its Taxonomy of Twenty Six Species in Rhododendron [J]. Scientia Silvae Sinicae, 2008, 44(2): 55-63.
[9]	Feng Liguo;Shao Dawei;Sheng Lixia;Zhao Lanyong;He Xiaodi. Pollen Morphology of Wild Rosa rugosa and Discussion on Its Origin and Evolution [J]. Scientia Silvae Sinicae, 2007, 43(12): 76-80.
[10]	Deng Bailuo;Tan Xiaofeng;Qi Longlin;He Jin;Hu Fangming. RAPD Analysis and Taxonomy of Sect. Camellia Species in Camellia [J]. Scientia Silvae Sinicae, 2006, 42(05): 36-41.
[11]	Guo Xianfeng;Wang Lianying;Yuan Tao. Study on Pollen Morphology of 4 Wild Herbaceous Peony [J]. Scientia Silvae Sinicae, 2005, 41(5): 184-186.
[12]	Qin Guofu;Zhao Jun;Liu Xiaoyong. CURRENT STATUS AND PROBLEMS OF MOLECULAR CLASSIFICATION IN THE PHYTOPLASMAS [J]. Scientia Silvae Sinicae, 2002, 38(6): 125-136.
[13]	Xu Huangcan;Yin Guangtian;Sun Qingpeng;Wu Jinkun. RESEARCH AND DEVELOPMENT OF RATTAN IN CHINA [J]. Scientia Silvae Sinicae, 2002, 38(2): 135-143.
[14]	Zhang Xingyao;Zhao Shiguang;Piao Chungen;Lü Quan;Jia Xiuzhen. MOLECULAR GENETIC DIVERSITY OF PATHOGENIC FUNGAL GROUP CAUSING TREE CANKER Ⅰ. ——EVIDENCE OF TAXA FOR DOTHIORELLA, DOTHICHIZA,CYTOSPORA AND CONIOTHYRIUM IN MOLECULAR LEVEL [J]. , 1999, 35(3): 34-40.
[15]	Zhang Xingyao. A STUDY ON THE TAXONOMY OF EXOBASIDIUM SPP．ACCORDING TO THE FUZZY ANALYSIS OF CULTURAL PROPERTIES AND THE ANALYSIS 0F 28S rDNA-PCR-RFLP [J]. , 1998, 34(4): 59-71.

Pollen Morphology of 20 Species in Picea by Scanning Electron Microscope

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments