落叶松枯梢病罹病叶及病梢真菌多样性

doi:10.11707/j.1001-7488.LYKX20230386

Abstract

Abstract:

Objective: This study aims to investigate the relationship between the differences in the diversity and community structure of endophytic fungi in the needles and shoots of Larix olgensis (larch) infected by Botryosphaeria laricina, and the incidence of larch dieback, so as to provide reference for the prevention of larch dieback. Method: Based on high-throughput sequencing technology, the differences in community structure, diversity and functional nutrient types of endophytic fungi between asymptomatic and diseased larch needles and shoots were analyzed by means of OTU number, identification group, diversity index and FUNGuild prediction. Result: The sequencing results showed that there were 1091 OTUs in needle samples and 1083 OTUs in shoot samples. The diversity of endophytic fungi in asymptomatic needles was significantly higher than that in the heavily infected needles, and there was no significant difference in endophytic fungal diversity between the asymptomatic and infected shoots. The dominant fungal phylum in needles and shoots was Ascomycetes, and the dominant order and genus in asymptomatic needles were Chaetothyriales and Arthrocatena, respectively. After infection with by B. laricina, the dominant order and genus in needles were Botryosphaeriales and Neofusicoccum, respectively, while Capnodiales and Microcyclosporella were the dominant fungal order and genus in shoots, respectively. LefSe analysis indicated that there were a total of 54 significant differential groups in fungal communities in needles and shoots. PCoA analysis further confirmed that the endophytic fungal communities of asymptomatic needles were significantly different from those of infected needles (anosim R=0.695, P < 0.05). The fungal nutritional types in needles were mainly pathological and saprophytic, and the pathological fungi were dominated after infection. The nutritional types of fungi in the shoots were mainly pathological and saprophytic. Conclusion: Compared with the shoots of L .olgensis, the endophytic fungal community structure in needle changes more significantly after infection by B. laricina, and the pathogen is enriched in the needles, thereby promoting the development of larch dieback.

Key words: Botryosphaeria laricina, needles, shoots, endophytic fungi, community structure

CLC Number:

S763.15

Xian Xie,Xun Deng,Liwen Song,Jun Liang. Endophytic Fungal Diversity in the Needles and Shoots Infected with Dieback of Larix olgensis[J]. Scientia Silvae Sinicae, 2024, 60(10): 76-85.

Figures/Tables 10

Table 1

Table 2

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

Fig.8

References 0

	白维晓, 孙玮宏, 肖瑞桐, 等. 两种不同白粉病抗性野生蔷薇内生真菌群落结构. 微生物学通报, 2019, 46 (6): 1300- 1308.
	Bai W X, Sun W H, Xiao R T, et al. Fungal endophytic communities of two wild Rosa species with different powdery mildew susceptibility. Microbiology China, 2019, 46 (6): 1300- 1308.
	陈健鑫, 唐　婕, 魏玉倩, 等. 云南野生春兰根系内生微生物多样性的分析. 微生物学报, 2023, 63 (1): 156- 169.
	Chen J X, Tang J, Wei Y Q, et al. Diversity of endophytic microbes in roots of wild Cymbidium goeringii in Yunnan. Acta Microbiologica Sinica, 2023, 63 (1): 156- 169.
	付晓霞, 王志明, 王选遥, 等. 落叶松落叶病和枯梢病合并防治研究. 吉林林业科技, 2016, 45 (2): 30- 34,40.
	Fu X X, Wang Z M, Wang X Y, et al. The combination contro research of Mycosphaerella laricis-leptolepiis and Botryosphaeria laricina. Journal of Jilin Forestry Science and Technology, 2016, 45 (2): 30- 34,40.
	刘满光, 张　娣, 路文雅, 等. 松枯梢病病原菌的研究进展. 中国农学通报, 2018, 34 (27): 97- 101. doi: 10.11924/j.issn.1000-6850.casb17110042
	Liu M G, Zhang D, Lu W Y, et al. Pathogen of pine shoot blight: research progress. Chinese Agricultural Science Bulletin, 2018, 34 (27): 97- 101. doi: 10.11924/j.issn.1000-6850.casb17110042
	刘秀嶶, 刘雪峰, 王　鹏, 等. 利用三种梢栖真菌生物防治落叶松枯梢病的研究. 林业科学研究, 2012, 25 (6): 685- 690. doi: 10.3969/j.issn.1001-1498.2012.06.002
	Liu X W, Liu X F, Wang P, et al. Biocontrolling larch shoot blight by using three species of dendrocola fungi. Forest Research, 2012, 25 (6): 685- 690. doi: 10.3969/j.issn.1001-1498.2012.06.002
	王桥美, 严　亮, 胡先奇, 等. 茶轮斑病对茶树叶片内生真菌群落结构的影响. 微生物学报, 2021, 61 (9): 2949- 2961.
	Wang Q M, Yan L, Hu X Q, et al. Effects of tea grey blight on the community structure of endophytic fungi in tea leaves. Acta Microbiologica Sinica, 2021, 61 (9): 2949- 2961.
	徐梅卿. 全国林业检疫性、危险性病害(病原微生物部分)(1). 林业科技通讯, 2023, (1): 1- 4.
	Xu M Q. National forestry quarantine and dangerous diseases (pathogenic microorganisms) (1). Forest Science and Technology, 2023, (1): 1- 4.
	张丽娜, 朱天辉, 杨佐忠, 等. 灰斑病对山茶叶部真菌群落的影响. 四川农业大学学报, 2011, 29 (3): 378- 385. doi: 10.3969/j.issn.1000-2650.2011.03.015
	Zhang L N, Zhu T H, Yang Z Z, et al. Influence of Camellia gray spot disease on foliar fungal communities. Journal of Sichuan Agricultural University, 2011, 29 (3): 378- 385. doi: 10.3969/j.issn.1000-2650.2011.03.015
	罗　鑫, 于　存. 贵州马尾松内生真菌多样性. 菌物学报, 2021, 40 (3): 531- 546.
	Luo X, Yu C. Diversity of endophytic fungi from Pinus massoniana in Guizhou Province, southwestern China. Mycosystema, 2021, 40 (3): 531- 546.
	谢　宪, 梁　军, 朱彦鹏, 等. 赤松纯林不同松枯梢病病级针叶的内生真菌多样性及群落结构. 林业科学, 2020, 56 (9): 51- 57. doi: 10.11707/j.1001-7488.20200906
	Xie X, Liang J, Zhu Y P, et al. Diversity and community structure of endophytic fungi in the pure forest of Pinus densiflora infectedby different incidences of Sphaeropsis sapinea. Scientia Silvae Sinicae, 2020, 56 (9): 51- 57. doi: 10.11707/j.1001-7488.20200906
	Berg G, Raaijmakers J M. Saving seed microbiomes. ISME Journal, 2018, 12, 1167- 70. doi: 10.1038/s41396-017-0028-2
	Bukamp J, Langer G, Langer E J. Sphaeropsis sapinea and fungal endophyte diversity in twigs of scots pine (Pinus sylvestris) in Germany. Mycological Progress, 2020, 19 (9): 985- 999. doi: 10.1007/s11557-020-01617-0
	Busby P E, Peay K G, Newcombe G. Common foliar fungi of Populus trichocarpa modify Melampsora rust disease severity. New Phytologist, 2016, 209 (4): 1681- 1692. doi: 10.1111/nph.13742
	Chapelle E, Mendes R, Bakker P A, et al. Fungal invasion of the rhizosphere microbiome. ISME Journal, 2016, 10, 265- 268.
	Chen D, Wang Y, Shi W, et al. Analysis of endophyte diversity of Rheum palmatum among different tissues and ages. Archives of Microbiology, 2022, 205 (1): 14.
	Cordovez V, Dini-Andreote F, Carrión V J, et al. Ecology and evolution of plant microbiomes. Annual Review of Microbiology, 2019, 73 (1): 69- 88. doi: 10.1146/annurev-micro-090817-062524
	Hardoim P R, Hardoim C C, van Overbeek L S, et al. Dynamics of seed-borne rice endophytes on early plant growth stages. PLoS ONE, 2012, 7 (2): e30438. doi: 10.1371/journal.pone.0030438
	Hattori Y, Ando Y, Nakashima C. Taxonomical re-examination of the genus Neofusicoccum in Japan. Mycoscience, 2021, 62 (4): 250- 259. doi: 10.47371/mycosci.2021.03.008
	Hu H T, Liu X Y, Zhang P L, et al. Novel secondary metabolites from the endobryophytic fungus Botrysphaeria laricina and their biological activity. Fitoterapia, 2020, 143, 104599.
	Nguyen N H, Song Z, Bates S T, et al. FUNGuild: an open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology, 2016, 20, 241- 248. doi: 10.1016/j.funeco.2015.06.006
	Pakvaz S, Soltani J. Endohyphal bacteria from fungal endophytes of the Mediterranean cypress (Cupressus sempervirens) exhibit in vitro bioactivity. Forest Pathology, 2016, 46 (6): 569- 581. doi: 10.1111/efp.12274
	Santangelo J S, Turley N E, Johnson M T. Fungal endophytes of Festuca rubra increase in frequency following longterm exclusion of rabbits. Botany, 2015, 93 (4): 233- 241. doi: 10.1139/cjb-2014-0187
	Sarsaiya S, Shi J S, Chen J S. A comprehensive review on fungal endophytes and its dynamics on Orchidaceae plants: Current research, challenges, and future possibilities. Bioengineered, 2019, 10 (1): 316- 334.
	Shade A, Jacques M A, Barret M. Ecological patterns of seed microbiome diversity, transmission, and assembly. Current Opinion in Microbiology, 2017, 37, 15- 22. doi: 10.1016/j.mib.2017.03.010
	Yang H, Liu X Y, Zhang P L, et al. New terpenoids and triketides from culture of the fungus Botrysphaeria laricina. Fitoterapia, 2020, 147, 104758. doi: 10.1016/j.fitote.2020.104758

处理 Treatment	丰富度指数 Chao1	多样性指数 Shannon	覆盖率 Coverage
无病斑针叶 Asymptomatic needles（AN）	660.96±31.21a	6.08±0.10a	0.99
染病较轻针叶 Lightly infected needles（LIN）	500.50±61.86ab	4.76±0.46ab	0.99
染病较重针叶 Heavily infected needles（HIN）	477.54±48.93b	3.90±0.49b	0.99

处理 Treatment	丰富度指数 Chao1	多样性指数 Shannon	覆盖率 Coverage
无病斑梢 Asymptomatic shoots（AS）	644.55±65.17a	4.34±0.40a	0.99
染病较轻梢 Lightly infected shoots（LIS）	571.50±33.98a	4.44±0.52a	0.99
染病较重梢 Heavily infected shoots（HIS）	503.71±38.09a	4.21±0.41a	0.99

[1]	Huan Xiao, Baiketuerhan Yeerjiang,Chunyu Zhang,Xiuhai Zhao. Relationship between Forest Layer Community Structure and Productivity of Broad-Leaved Korean Pine Forest in Changbai Mountain [J]. Scientia Silvae Sinicae, 2024, 60(3): 57-64.
[2]	Sen Xu,Yawen Dong,Shuanglin Chen,Ziwu Guo,Liting Yang,Yanyan Xie. Characteristics of Carbon and Nitrogen Metabolism in Sheath Leaves and Its Relationship with Taste Quality of Phyllostachys violascens 'Prevernalis' Shoots under Mulching [J]. Scientia Silvae Sinicae, 2022, 58(8): 109-116.
[3]	Zhi Li,Xiaoyan Xue,Zhen Liu,Qifei Cai,Xiaodong Geng,Jian Feng,Huina Zhou,Tao Zhang,Mingwan Li,Yanmei Wang. Analysis of Bacterial Community Structure, Diversity and Functional Prediction in Different Organs of Healthy and Diseased Idesia polycarpa Plants [J]. Scientia Silvae Sinicae, 2022, 58(8): 136-148.
[4]	Bin Li,Hongxiang Shi,Lanlan Liu,Pu Yang,Xin Zhang,Hang Chen,Ying Feng,Xiaoming Chen. Characteristics of Community Structure and Functional Group of Fungi in Leaf, Stem, Root and Rhizosphere Soil of Ligustrum lucidum [J]. Scientia Silvae Sinicae, 2022, 58(5): 102-112.
[5]	Lixiang Wang,Shupeng Cui,Lulu Kong,Xuan Wang,Zongji Yang,Lili Ren,Youqing Luo. Inhibitory Effects of Endophytic Fungi in Host Tree on the Symbiotic Fungi of Sirex nitobei (Hymenoptera: Siricidae) [J]. Scientia Silvae Sinicae, 2022, 58(5): 113-120.
[6]	Wei Cong,Jingjing Yu,Haimang Yu,Yi Ding,Yuguang Zhang. Diversity and Community Assembly of Forest Soil Microorganisms in Different Climatic Zones [J]. Scientia Silvae Sinicae, 2022, 58(2): 70-79.
[7]	Yongzhong Chen,Caixia Liu,Yanming Xu,Zhen Zhang,Yinghe Peng,Longsheng Chen,Yirong Su,Rui Wang,Wei Tang. Effects of Herbage Inter-Planting on Structure and Stability of Soil Microbial Community in Camellia oleifera Plantations [J]. Scientia Silvae Sinicae, 2022, 58(11): 61-70.
[8]	Pengyu Zhao,Xue Bai,Pingmei Yan,Xiaodong Zhao,Xiaoying Wu,Baofeng Chai. Responses of Soil Bacterial Community Structure and Phenotype to Soil Heterogeneity in Larix principis-rupprechtii Forest [J]. Scientia Silvae Sinicae, 2021, 57(7): 101-110.
[9]	Rongrong Pang,Jieying Peng,Yan Yan. Factors Influencing Aboveground Biomass in the Secondary Forest of Quercus aliena var. acutiserrata in Taibai Mountain [J]. Scientia Silvae Sinicae, 2021, 57(10): 157-165.
[10]	Xian Xie, Jun Liang, Yanpeng Zhu, Ruirui Hu, Yuan Cheng, Xingyao Zhang. Diversity and Community Structure of Endophytic Fungi in the Pure Forest of Pinus densiflora Infected by Different Incidences of Sphaeropsis sapinea [J]. Scientia Silvae Sinicae, 2020, 56(9): 51-57.
[11]	Changlin Liu,Guoying Zhou,Bai Xiao,Jun Liu. Diversity of Endophytic Fungi in Heartwood and Sapwood of Dalbergia odorifera [J]. Scientia Silvae Sinicae, 2020, 56(4): 109-120.
[12]	Cao Hongyu, Gao Guanglei, Ding Guodong, Zhang Ying, Zhao Yuanyuan, Ren Yue, Chen Yuxuan, Guo Mishan. Community Structure and Diversity of Soil Fungi in Four Habitats in Hulun Buir Sandy Land [J]. Scientia Silvae Sinicae, 2019, 55(8): 118-127.
[13]	Fang Jiaxing, Wu Chengxu, Lu Wenjuan, Liu Fu, Zhang Sufang, Zhang Zhen, Kong Xiangbo. Correlation Analysis between the Shoot Damages and Trap Catches of Tomicus minor in the Pinus yunnanensis Forest [J]. Scientia Silvae Sinicae, 2019, 55(4): 129-135.
[14]	Wang Haoyun, Wu Feng, Zhu Xiaokun, Xie Weibin. Effects of Leaf Types on Growth and Chlorophyll Fluorescence Characteristics in Pinus massoniana Seedlings [J]. Scientia Silvae Sinicae, 2019, 55(3): 183-192.
[15]	Chen Jinlei, Fang Xi, Gu Xiang, Li Leida, Liu Zhaodan, Wang Liufang, Zhang Shiji. Composition, Structure, and Floristic Characteristics of Two Forest Communities in the Central-Subtropical China [J]. Scientia Silvae Sinicae, 2019, 55(2): 159-172.

Endophytic Fungal Diversity in the Needles and Shoots Infected with Dieback of Larix olgensis

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 0

Related Articles 15

Recommended Articles

Metrics

Comments