Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (9): 98-105.doi: 10.11707/j.1001-7488.20220910
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Jing Cao1,2,Xingjiang Qi1,3,Xiliang Zheng1,Zheping Yu1,Shuwen Zhang1,Haiying Ren1,3,*
Received:
2021-06-05
Online:
2022-09-25
Published:
2023-01-18
Contact:
Haiying Ren
CLC Number:
Jing Cao,Xingjiang Qi,Xiliang Zheng,Zheping Yu,Shuwen Zhang,Haiying Ren. Changes of Physiology and Gene Expression during Seed Germination of Myrica rubra[J]. Scientia Silvae Sinicae, 2022, 58(9): 98-105.
Table 1
Specific primers of related genes used for qRT-PCR"
基因名称Gene names | 同源基因Homolog | 引物序列Primer sequences(5′~3′) | 产物大小Product size/bp |
MrUBQ1 | MRNA_021433_1 | F: AAGGCGAAGATCCAAGACAA | 116 |
R: GTGGAGCGTCGACTCTTTCT | |||
MAPKKK15 | MRNA_011497_1 | F: ACGTCCCCATGTACAACCTT | 172 |
R: TTGATGTCGCAATGCACCAA | |||
YMCIbHLH1 | MRNA_012119_1 | F: GACGGAGATAAGGGGAAGGG | 152 |
R: TTGCCCTCCACCTTATCGTT | |||
TUA2 | MRNA_012224_1 | F: TGTCCTGTCAACCCACTCTC | 216 |
R: ATCGGAGGCAAGACAACTGA | |||
PHYA | MRNA_022168_1 | F: GTCCCTCTCTCGGATCCATG | 168 |
R: TGTCTCGGCCTTCCTTCAAT | |||
MAPKKK18 | MRNA_022233_1 | F: TAGGTCATGGAAGCTTCGCA | 151 |
R: CAACTCACGAGCCGATCAAG | |||
FOA1 | MRNA_000845_1 | F: CTTTCATTGCCCACCACCTC | 153 |
R: CGACAAGCGCTTCATGATCA | |||
YMSQS1 | MRNA_024255_2 | F: TTCTTCGAGCCCTTGACACT | 192 |
R: TGGTCCCGTTCAAGAATCCA | |||
RVE1 | MRNA_003978_1 | F: ACAGAGTCTCACAGGCCATC | 179 |
R: CAGAGCGCACCATGTACTTC | |||
WRKY6 | MRNA_023462_1 | F: ATCGCCCAGGAACCTTAACA | 183 |
R: TTCCTCATCGTGGCTTCAGT | |||
HD2B | MRNA_005241_1 | F: AGGGATGATGGAGGCTATGC | 181 |
R: TTTCCAGTCGACTCAGGCTT | |||
YMBZIP | MRNA_002903_2 | F: AGACGGCCTACTTTTGGTGA | 183 |
R: CTGCACTGAAGAGGACTGGA | |||
MAPKKK14 | MRNA_012448_1 | F: AGGGGTAAACTGGGAATCGG | 216 |
R: TCCAAACGTGTCCTCCTCTC |
Fig.1
The rules of enzyme activities during bayberry germination 1, 2, 3, 4, 5 of the abscissa represent the five stages of the seed germination process of Myrica rubra, indicating that the newly sown seeds and young roots are 0~1 cm, 1~2 cm, 2~3 cm and longer than 3 cm, respectively. Different letters on the error line indicate significant differences between treatments (P < 0.05)."
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