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Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (9): 79-89.doi: 10.11707/j.1001-7488.20220908

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Nano Silver and Ethephon Pretreatment Regulates the Quality of Cut Flowers of Itoh Peony 'Bartzella'

Guoan Shi1,Yi Wang1,2,Tian Shi1,3,Shuangcheng Gao1,Yuan Zhao1,Shenshen Shang1,Siyuan Hu1   

  1. 1. Luoyang Key Laboratory of Peony Biology, College of Mudan, Henan University of Science and Technology Luoyang 471011
    2. Zhengzhou Antu Bioengineering Co., Ltd Zhengzhou 450000
    3. Luoyang National Peony Garden Luoyang 471011
  • Received:2021-09-07 Online:2022-09-25 Published:2023-01-18

Abstract:

Objective: Itoh peony 'Bartzella' is a popular type of cut flowers among consumers. In this study, fresh cut flowers of Itoh peony were used to study the physiological effect of ethylene induced senescence in order to provide a theoretical basis for the regulating technology of fresh-keeping. Method: The 'Bartzella' cut flowers were exogenously pretreated with 20 μg·L-1 nano silver (NS), 5 and 20 μL·L-1 2-chloro-2-ethyl phosphoric acid (CEPA, ethephon) for one hour, respectively. The flowering and senescence characteristics of 'Bartzella' cut flowers were observed by inserting the flowers in vase. The dynamic changes of ethylene release from petals were measured by gas chromatography. The water status of flower branches was measured by weighing method. The membrane lipid peroxidation level was determined by spectrophotometry and the energy state was determined by HPLC. With principal component analysis, the relationship between ethylene metabolism, lipid peroxidation and energy state was analyzed. Result: 1) The results showed that cut flowers of 'Bartzella' absorbed water rapidly, reached full bloom in 24 h after vase inserting, and the vase life was only 3~4 days. The vase life was able to be significantly prolonged in the presence of NS, and the vase quality was improved. However, CEPA accelerated the senescence process of cut flowers, and had a significant dose effect. 2) The cut flowers in vase for 6 h had the characteristics of ethylene climacteric peak. Compared with the control, the ethylene release peak of NS pretreatment decreased by 26.95%, while CEPA significantly promoted the ethylene release, and 5 and 20 μL·L-1 CEPA increased by 11.52% and 125.50%, respectively. 3) From opening to senescence, 'Bartzella' cut flowers experienced a process of water imbalance and membrane lipid peroxidation intensification of flower branches. NS was able to improve the water status of flower branches, inhibit the increase of MDA content and the decrease of soluble protein content. CEPA had the opposite effect with nano silver. 4) NS promoted the transformation and accumulation of active substrates and improved the activity and energy charge level of key enzymes in respiratory metabolism of petals. However, the effect of CEPA was opposite to that of NS for cut flowers. CEPA decreased the content of respiratory substrates, the activity of key enzymes in respiratory metabolism and the level of energy charge. Conclusion: The cut flower of Itoh peony 'Bartzella' belongs to an ethylene hypersensitive type. From opening to senescence process, 'Bartzella' cut flower is jointly regulated by ethylene, water and energy metabolisms. This study suggests that controlling ethylene and energy metabolisms should be a basis strategy to improve the quality of cut flowers of Itoh peony 'Bartzella'.

Key words: Itoh peony, 'Bartzella', cut flower, nano silver, ethephon, senescence characteristics, metabolism, regulation effect

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